Purpose To explore the pathogenesis of venous thromboembolism (VTE) and provide bioinformatics basis for the prevention and treatment of VTE. Methods The R software was used to obtain the gene expression profile data of GSE19151, combining with the CIBERSORT database, obtain immune cells and differentially expressed genes (DEGs) of blood samples of VTE patients and normal control, and analyze DEGs for GO analysis and KEGG pathway enrichment analysis. Then, the protein-protein interaction (PPI) network was constructed by using the STRING database, the key genes (hub genes) and immune differential genes were screened by Cytoscape software, and the transcription factors (TFs) regulating hub genes and immune differential genes were analyzed by the NetworkAnalyst database. Results Compared with the normal group, monocytes and resting mast cells were significantly expressed in the VTE group, while regulatory T cells were significantly lower. Ribosomes were closely related to the occurrence of VTE. 10 hub genes and immune differential genes were highly expressed in VTE. MYC, SOX2, XRN2, E2F1, SPI1, CREM and CREB1 can regulate the expressions of hub genes and immune differential genes. Conclusions Ribosomal protein family genes are most relevant to the occurrence and development of VTE, and the immune differential genes may be the key molecules of VTE, which provides new ideas for further explore the pathogenesis of VTE.

中文翻译：

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静脉血栓栓塞症的免疫学分析及差异基因筛查

目的探讨静脉血栓栓塞症（VTE）的发病机制，为VTE的防治提供生物信息学基础。方法利用R软件获取GSE19151基因表达谱数据，结合CIBERSORT数据库，获取VTE患者和正常对照血样的免疫细胞和差异表达基因（DEGs），分析DEGs进行GO分析和KEGG通路富集分析。然后，利用STRING数据库构建蛋白质-蛋白质相互作用（PPI）网络，通过Cytoscape软件筛选关键基因（枢纽基因）和免疫差异基因，以及调控枢纽基因和免疫差异基因的转录因子（TFs）由 NetworkAnalyst 数据库分析。结果与正常组相比，单核细胞和静息肥大细胞在 VTE 组中显着表达，而调节性 T 细胞显着降低。核糖体与VTE的发生密切相关。10个枢纽基因和免疫差异基因在VTE中高表达。MYC、SOX2、XRN2、E2F1、SPI1、CREM和CREB1可以调节枢纽基因和免疫差异基因的表达。结论核糖体蛋白家族基因与VTE的发生发展最为相关，免疫差异基因可能是VTE的关键分子，为进一步探讨VTE的发病机制提供了新的思路。SPI1、CREM和CREB1可以调节枢纽基因和免疫差异基因的表达。结论核糖体蛋白家族基因与VTE的发生发展最为相关，免疫差异基因可能是VTE的关键分子，为进一步探讨VTE的发病机制提供了新的思路。SPI1、CREM和CREB1可以调节枢纽基因和免疫差异基因的表达。结论核糖体蛋白家族基因与VTE的发生发展最为相关，免疫差异基因可能是VTE的关键分子，为进一步探讨VTE的发病机制提供了新的思路。