The availability of zinc has been correlated with reproductive performance. To validate the hypothesis, a 60‐day feeding trial was conducted to evaluate the effect of dietary zinc on reproductive performance and gonadotropin gene (FSH and LH) expression in threatened Clarias magur brooder. Four isonitrogenous (35%) and iso‐energetic (372.18 ± 1.73 k cal/100 g) diets were prepared with zinc acetate as control (100 mg/kg diet normal requirement), T1 (50 mg/kg diet, low zinc), T2 (200 mg/kg diet, medium zinc) and T3 (300 mg/kg diet, high zinc). The weight gain % and specific growth rate were significantly (p < 0.05) decreased with the increased dietary zinc level. The pituitary mRNA levels of FSH were assessed during the reproductive cycle of female C. magur, and the FSH expression was increased which was significantly (p < 0.05) highest in T1 group. Similarly, LH expression was revealed to be highest. In all treatment groups except T1, GTH expression reduced significantly by increasing zinc inclusion in the diet. GSI and fecundity were lowest during initial sampling and start to increase was observed. The highest GSI and fecundity were recorded in the T1; however, GSI and fecundity significantly (p < 0.05) reduced with increasing the zinc level. Reproductive parameters like spawning fecundity, fertilization rate, hatching rate and larval survival were found to be dose‐dependent of zinc. This revealed that 50 mg/kg zinc was optimum for enhanced reproductive performance; therefore, the present study proposed that 50 mg/kg of zinc could be supplemented for flexible enhanced reproductive performance in threatened Asian catfish, C. magur.

中文翻译：

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日粮锌对濒临灭绝的亚洲Cl鱼（Clarias magur（Hamilton，1822）亲鱼）生殖性能和促性腺激素（FSH和LH）表达的调节作用

锌的可用性已与生殖性能相关。为验证该假设，进行了为期60天的喂养试验，以评估日粮锌对濒危克拉格犬（Clarias magur）育雏者生殖性能和促性腺激素基因（FSH和LH）表达的影响。分别以乙酸锌作为对照（100 mg / kg饮食的正常需求），T1（50 mg / kg饮食，低锌），4种等氮（35％）和等能量（372.18±1.73 k cal / 100 g）饮食， T2（200 mg / kg饮食，中等锌）和T3（300 mg / kg饮食，高锌）。 随着饮食中锌含量的增加，体重增加％和比生长速率显着降低（p <0.05）。在女性C的生殖周期中评估了FSH的垂体mRNA水平。 magur，并且FSH表达增加， 在T1组中最高（p <0.05）。类似地，LH表达被揭示是最高的。在所有治疗组中，除T1以外，通过增加饮食中的锌含量，GTH的表达均显着降低。在初始采样期间，GSI和繁殖力最低，并开始增加。T1记录了最高的GSI和繁殖力；然而，GSI和生殖力显着（p <0.05）随着锌含量的增加而降低。生殖参数，如产卵量，受精率，孵化率和幼虫存活率，均与锌的剂量有关。这表明50 mg / kg锌是增强生殖性能的最佳选择。因此，目前的研究提出，50mg / kg的锌可以补充在威胁亚鲶鱼，挠性增强的繁殖性能Ç。 玛古尔。