Azoxystrobin, a widely used broad-spectrum strobilurin fungicide, may pose a potential threat in agricultural ecosystems. To assess the ecological risk of azoxystrobin in real soil environments, we performed a study on the toxic effects of azoxystrobin on earthworms (Eisenia fetida) in three different natural soils (fluvo-aquic soil, black soil and red clay soil) and an artificial soil. Acute toxicity of azoxystrobin was determined by filter paper test and soil test. Accordingly, exposure concentrations of chronic toxicity were set at 0, 0.1, 1.0 and 2.5 mg kg⁻¹. For chronic toxicity test, reactive oxygen species, activity of antioxidant enzymes (superoxide dismutase, catalase and peroxidase), detoxifying enzyme (glutathione transferase), level of lipid peroxidation (malondialdehyde) and level of oxygen damage of DNA (8-hydroxydeoxyguanosine) in earthworms were determined on the 7th, 14th, 21st, 28th, 42nd and 56th days after treatment. Both acute and chronic toxic results showed azoxystrobin exhibit higher toxicity in natural soil than in artificial soil, indicating that traditional artificial soil testing method underestimate ecotoxicity of azoxystrobin in a real agricultural environment on the earthworm population. Combining with the analysis of soil physicochemical properties, the present experiment provided scientific guidance for rational application of azoxystrobin in agricultural production systems.

嘧菌酯是一种广谱的球型伞菌素杀菌剂，可能对农业生态系统构成潜在威胁。为了评估实际土壤环境中嘧菌酯的生态风险，我们进行了一项研究，研究了嘧菌酯对三种不同天然土壤（潮土，黑土和红粘土）和人造土壤中worm（Eisenia fetida）的毒性作用。。通过滤纸试验和土壤试验确定了嘧菌酯的急性毒性。因此，慢性毒性的暴露浓度设定为0、0.1、1.0和2.5mg kg ^-1。对于慢性毒性测试，reactive中的活性氧种类，抗氧化酶的活性（超氧化物歧化酶，过氧化氢酶和过氧化物酶），解毒酶（谷胱甘肽转移酶），脂质过氧化的水平（丙二醛）和DNA中的氧破坏水平（8-羟基脱氧鸟苷）在治疗后第7、14、21、28、42和56天进行测定。急性和慢性毒性结果均显示，天生的土壤中嘧菌酯的毒性要高于人工土壤，这表明传统的人工土壤测试方法低估了在真实的农业环境中a虫的生态毒性。结合对土壤理化性质的分析，