Combining electrochemiluminescence (ECL) with nanozyme amplification provides unique advantages for the detection of antibiotic residues. Herein, a molecularly imprinted chloramphenicol (CAP) sensor was established based on aggregation-induced (AI)-ECL and nanozyme amplification. Covalent organic framework materials with AI-ECL groups (COF-AI-ECL) and nanozyme Co₃O₄ were synthesised as the signal element and the amplification element, respectively. Subsequently, using CAP as a template molecule, a molecularly imprinted polymer (MIP) was fabricated on the electrode surface modified with COF-AI-ECL and Co₃O₄. The ECL signal of COF-AI-ECL was catalytically amplified by Co₃O₄, whereas CAP effectively quenched this signal. Consequently, the ECL signal was controlled by the elution and adsorption of CAP by the MIP, thus establishing a new method for CAP detection. Unlike traditional ECL reagent, COF-AI-ECL exhibited a stable and strong ECL signal. Therefore, COF-AI-ECL in combination with the MIP provided greater sensitivity and enhanced selectivity. The linear range of the developed CAP sensor was 5 × 10⁻¹³ to 4 × 10⁻¹⁰ mol/L, with a detection limit of 1.18 × 10⁻¹³ mol/L. Moreover, the recoveries range of 85.0%–106.2% were obtained for the detection of CAP in real honey, milk, and chicken samples, indicating the potential of this sensor design for the detection of trace antibiotic residues in food safety applications.

将电化学发光（ECL）与纳米酶扩增结合在一起，可为检测抗生素残留提供独特的优势。本文中，基于聚集诱导的（AI）-ECL和纳米酶扩增建立了分子印迹氯霉素（CAP）传感器。分别合成了具有AI-ECL基团的共价有机骨架材料（COF-AI-ECL）和纳米酶Co ₃ O ₄作为信号元素和放大元素。随后，使用CAP作为模板分子，在用COF-AI-ECL和Co ₃ O ₄改性的电极表面上制备了分子印迹聚合物（MIP）。Co ₃ O ₄催化放大COF-AI-ECL的ECL信号，而CAP有效地消除了该信号。因此，通过MIP对CAP的洗脱和吸附来控制ECL信号，从而建立了一种新的CAP检测方法。与传统的ECL试剂不同，COF-AI-ECL表现出稳定而强大的ECL信号。因此，COF-AI-ECL与MIP结合使用可提供更高的灵敏度和更高的选择性。研发的CAP传感器的线性范围为5×10 ^-13至4×10 ^-10  mol / L，检测极限为1.18×10 ^-13  mol / L。此外，用于检测真实蜂蜜，牛奶和鸡肉样品中CAP的回收率达到85.0％–106.2％，表明该传感器设计在食品安全应用中检测痕量抗生素残留的潜力。