The outbreaks caused by the Aedes aegypti-transmitted dengue virus (DENV), zika virus (ZIKV), and chikungunya virus (CHIKV) result in a significant impact to the health systems of tropical countries. Furthermore, the occurrence of patients coinfected by at least two of these arboviruses is an aggravating factor in that scenario. On this basis, surveillance tools such as the Rapid Index Survey for Aedes aegypti (LIRAa) are used to estimate vector infestation in order to improve the prediction of human outbreaks. Ae. aegypti eggs were collected in the city of Vitória da Conquista, in Bahia State, Brazil, and subsequently hatched into larvae, which were analyzed in pools or individually for the presence of DENV, ZIKV, and CHIKV by molecular biology methods. The detection data for arboviruses were crossed with the LIRAa obtained in each region of the study city. Thirty larvae pools were analyzed, and fourteen (46.6%) of them were detected positive for DENV, ZIKV, and/or CHIKV. Among the individually analyzed larvae (n=30), nine (30%) were positive for any of these arboviruses, and four (13.3%) were simultaneously coinfected by DENV and ZIKV. Furthermore, there was a positive correlation between the detection of circulating arboviruses and LIRAa. The simultaneous Ae. aegypti larvae infection by two different arboviruses is an unprecedented finding. This result suggests the occurrence of a vertical arboviruses co-transmission from the female mosquito to its offspring in nature. The occurrence of concomitant circulation of DENV, ZIKV, and CHIKV in Ae. aegypti from a single study region is another finding of this article. Finally, LIRAa seems to not only estimate vector infestation but also to predict circulation of arboviruses.

造成疫情大爆发的埃及伊蚊在热带国家的卫生系统显著影响-transmitted登革热病毒（DENV），寨卡病毒（ZIKV）和基孔肯雅病毒（CHIKV）的结果。此外，在这种情况下，至少有两种这种虫媒病毒并发感染的患者的出现是一个加重因素。在此基础上，使用诸如埃及伊蚊快速指数调查（LIRAa）之类的监视工具来估计病媒侵染，以改善对人类暴发的预测。e 埃及在巴西巴伊亚州的Vitóriada Conquista市收集卵，然后将其孵化成幼虫，然后通过分子生物学方法在池中或单独分析其幼虫中是否存在DENV，ZIKV和CHIKV。虫媒病毒的检测数据与在研究城市的每个地区获得的LIRAa交叉。分析了三十个幼虫池，其中十四个（46.6％）被检测出DENV，ZIKV和/或CHIKV阳性。在单独分析的幼虫中（n = 30），其中9种（30％）对这些虫媒病毒均为阳性，而DENV和ZIKV同时感染了4种（13.3％）。此外，循环虫媒病毒的检测与LIRAa之间存在正相关。同时Ae。埃及两种不同虫媒病毒对幼虫的感染是前所未有的发现。这一结果表明，垂直虫媒病毒在自然界中是从雌性蚊子向后代共传播的。Ae中DENV，ZIKV和CHIKV伴随循环的发生。来自单个研究区域的埃及是本文的另一个发现。最后，LIRAa似乎不仅可以估计载体的侵染，而且可以预测虫媒病毒的传播。