Abstract

As an ornamental organ, petal plays an important role in attracting insect pollination, it’s development attracts the attention of many researchers. In this study, a sunflower mutant line with ligulate-like tubular petal (lpm) was screened out, and its disc tubular florets were characterized with elongated ligulate-like petals, longer filaments size, fewer anthers and lower seed setting rate, compared with wide type (WT) plants. From the suppression subtractive hybridization library constructed using inflorescence of lpm and WT plants, the HaYABBY was identified with expressing differentially. The cDNA and structural sequences of HaYABBY gene were 997 and 4339 bp, respectively, encoding a peptide with 219 amino acids. The analysis of HaYABBY expression showed that it presented higher transcription level in flower than root, stem and leaf of sunflower. The expression level of YABBY in disc florets of lpm was significantly higher than those of WT, especially at early floral developmental stage. The results suggested that HaYABBY could impact the floral development at early stage, particularly on petal elongation and floral symmetry morphogenesis in sunflower. Furthermore, the results implied that HaYABBY would be involved in the regulation of reproductive system development and affect their fertility. Our findings will provide some insight to understand the regulating network of floral development in sunflower, and help to cultivate new sunflower varieties with high ornamental value.

中文翻译：

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HaYABBY基因与向日葵的类似带状管状花瓣突变植物的花期发育有关。

摘要

花瓣作为观赏器官，在吸引昆虫授粉中起着重要作用，它的发展引起了许多研究人员的关注。在这项研究中，筛选出向日葵的突变体系，其具有结扎状管状花瓣（lpm），其圆盘状管状小花的特征是细长的结扎状花瓣，较长的花丝大小，较少的花药和较低的结实率，而较宽类型（WT）植物。从使用lpm和WT植物的花序构建的抑制性消减杂交文库中，鉴定出HaYABBY具有差异表达。HaYABBY的cDNA和结构序列该基因分别为997和4339 bp，编码具有219个氨基酸的肽。HaYABBY表达的分析表明，它在花中的转录水平高于向日葵的根，茎和叶。YABBY在lpm圆盘小花中的表达水平显着高于WT，特别是在花发育早期。结果表明，HaYABBY可能影响早期的花发育，特别是影响向日葵的花瓣伸长和花对称形态。此外，结果暗示HaYABBY将参与生殖系统发育的调节并影响其生育能力。我们的发现将为理解向日葵花卉发育的调控网络提供一些见识，并有助于培育具有较高观赏价值的向日葵新品种。