Abstract

Three intergenic spacers (psbA–trnH, trnL–trnF, trnS–trnG) of chloroplast DNA (cpDNA) and an internal transcribed spacer of nuclear ribosomal DNA (ITS rDNA) were used to study the genetic diversity and phylogenetic relationships of the species Oxytropis czukotica, O. exserta, O. gorodkovii, O. kamtschatica, O. mertensiana, O. nigrescens, O. pumilio, O. revoluta, and O. susumanica of the section Arctobia of the genus Oxytropis. According to cpDNA data, most populations are characterized by a low and medium haplotype (h varies from 0.154 to 0.583) and low nucleotide (π varies from 0.0002 to 0.0050) diversity. An analysis of the genealogical relationships of chlorotypes showed a clear separation of the studied taxa and the genetic proximity of O. nigrescens to O. susumanica and O. kamtschatica to O. exserta, the last two being the most diverged from all the others. In O. czukotica, three ITS rDNA ribotypes were detected; in O. nigrescens and O. susumanica, one common ribotype was found; and in all the others, one individual ribotype was identified for each species. According to the data of nucleotide polymorphism of markers of two genomes, the status of O. czukotica, O. gorodkovii, and O. pumilio taxa as a three separate species was confirmed. We suggest that O. susumanica is an intraspecific taxon of O. nigrescens. The revealed genetic similarities and differences of O. revoluta, O. exserta, and O. kamtschatica and their phylogenetic relationships do not correspond to the division of the Arctobia section into subsections; therefore, additional comprehensive studies are needed.

中文翻译：

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根据叶绿体基因间隔子和核基因组ITS序列分析东北亚嗜氧菌断面弧菌的亲缘关系

摘要

使用三个叶绿体DNA（cpDNA）的基因间隔子（psbA–trnH，trnL–trnF，trnS–trnG）和核糖体DNA的内部转录间隔子（ITS rDNA）来研究捷克嗜氧菌的遗传多样性和系统发育关系，O.缘桉，O. gorodkovii，O. kamtschatica，O. mertensiana，O.变黑，O. pumilio，O. REVOLUTA，和O. susumanica截面的Arctobia属的棘。根据cpDNA数据，大多数种群的特征是低和中等单倍型（h 在0.154至0.583之间变化）和低核苷酸（π在0.0002至0.0050之间变化）多样性。对叶绿体类型的族谱关系的分析表明，已研究的生物分类明显分离，而O. nigrescens与O. susumanica和O. kamtschatica与O. exserta的遗传接近性，最后两个之间的差异最大。在O. czukotica中，检测到了三种ITS rDNA核糖型。在O.变黑和O. susumanica，发现一种常见的核糖型；在所有其他物种中，为每个物种鉴定了一个单独的核糖型。根据两个基因组标记的核苷酸多态性数据，确认了O. czukotica，O。gorodkovii和O. pumilio类群是三个独立的物种。我们建议O. susumanica是O. nigrescens的种内分类群。揭示的O. revoluta，O. exserta和O. kamtschatica的遗传相似性和差异及其系统发生关系不符合Arctobia的划分。分成小节；因此，需要进行额外的综合研究。