Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO - 3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.

中文翻译：

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从雪松和黑松中新分离出的孢粉素微笼作为奥沙利铂的载体；基于 xCELLigence RTCA 的释放检测

由于其理想的物理化学和生物学特性，孢粉素介导的控制药物递送已被广泛研究。在本文中，孢粉素从 C. libani 和 P. nigra 花粉中成功提取，然后加载众所周知的抗癌药物奥沙利铂。通过使用光学显微镜、FT-IR、SEM 和 TGA 确认载药量和理化特征。首次使用实时细胞分析仪系统 xCELLigence 实时记录加载奥沙利铂的孢粉素介导的细胞死亡（CaCo-2 和 Vero 细胞）。两种释放试验都证实奥沙利铂从孢粉中缓慢释放约 40-45 小时。MYC 和 FOXO-3 基因在 CaCo2 细胞中的表达显着增加，非癌性 Vero 细胞减少，证实了孢粉质介导的奥沙利铂控制释放促进细胞凋亡，防止负面影响扩散到附近健康细胞的事实。所有结果表明，C. libani 和 P. nigra 可以作为药物缓慢递送的合适候选者。