Parasitic loranthus [Taxillus chinensis (DC.) Danser] is an important medicinal plant that produces recalcitrant seeds that are sensitive to dehydration. Desiccation tolerance is critical for the survival of recalcitrant seeds in low-moisture environments. Clarifying how these seeds respond to desiccation is important for long-term conservation. Thus, the viability, germination, microstructure, and antioxidant enzyme activities of dehydrated parasitic loranthus seeds were investigated. Diverse organelles were degraded or deformed during dehydration treatments. Additionally, superoxide dismutase and catalase activities gradually decreased in response to desiccation stress. A proteomic analysis involving TMT-labeling and LC–MS/MS were performed. A total of 1479 proteins were identified, of which 141 were differentially expressed proteins (DEPs) at 16 and 36 h after initiating the dehydration treatments. A functional annotation based on gene ontology revealed that the DEPs were mainly localized in chloroplasts and were related to energy metabolism, responses to stimuli, and the regulation of biological processes. A KEGG pathway enrichment analysis determined that several of the identified proteins were associated with signal transductions, photosynthesis, and glycolysis/gluconeogenesis. The results suggest that the efficient removal of excessive ROS amounts may be crucial for promoting parasitic loranthus seed germination under dehydration stress conditions. A series of candidate dehydration stress-related proteins were identified and may be relevant for enhancing the dehydration tolerance of the recalcitrant seeds. To the best of our knowledge, this is the first study to elucidate the possible molecular mechanisms underlying the sensitivity of recalcitrant parasitic loranthus seeds to dehydration via a proteomic analysis involving TMT-labeling.

寄生桑寄生[钝果寄生属红豆杉（DC。）Danser]是一种重要的药用植物，其产生对脱水敏感的顽calc种子。干燥耐受性对于低水分环境中顽calc性种子的生存至关重要。澄清这些种子对干燥的反应方式对于长期保存很重要。因此，研究了脱水寄生狼牙种子的活力，发芽，微结构和抗氧化酶活性。脱水处理过程中多种细胞器降解或变形。另外，响应于干燥胁迫，超氧化物歧化酶和过氧化氢酶活性逐渐降低。进行了蛋白质组学分析，包括TMT标记和LC-MS / MS。总共鉴定出1479种蛋白质，在开始脱水处理后的16和36小时，其中141个是差异表达蛋白（DEP）。基于基因本体论的功能注释显示，DEP主要位于叶绿体中，并且与能量代谢，对刺激的反应以及生物过程的调节有关。KEGG途径富集分析确定了几种已鉴定的蛋白质与信号转导，光合作用和糖酵解/糖异生有关。结果表明，在脱水胁迫条件下，有效去除过量的ROS对促进寄生狼牙种子萌发至关重要。鉴定了一系列候选的与脱水胁迫相关的蛋白，这些蛋白可能与增强顽it性种子的脱水耐受性有关。