Through applying the mixed-ligand method, a new coordination polymer (CP) of Cu(II) with the chemical formula of {Cu₅(L)₂(1,4-bimb)(μ₃-OH)₂(H₂O)₈]·2H₂O}_n (1, 1,4-bimb = 1,4-bis(imidazol-1-ylmethyl)benzene and H₄L = 1,2,4,5-cyclohexanetetracarboxylic acid) was synthesized under the solvothermal conditions. Furthermore, the biological effect of compound against glomerulonephritis therapy was detected, and the details mechanism was investigated. First of all, the mesangial cells proliferation was detected through using the Cell Counting Kit-8 (CCK-8) method. Then, the Annexin V-FITC/PI method was utilized to determine cell cycle arrest and the apoptosis rate. Next, the caspase-8 and caspase-3 expression level in mesangial cells was detected via applying the western blot method. Finally, the inflammatory cytokines released into the plasma was assessed by ELISA detection kit.

通过与化学式施加Cu的混合配位体的方法，一种新的配位聚合物（CP）（II）{的Cu ₅（L）₂（1,4-BIMB）（μ ₃ -OH）₂（H ₂ ö ）₈ ]·2H ₂ ö} _ñ（1，1,4- BIMB = 1,4-双（咪唑-1-基甲基）苯和H ₄在溶剂热条件下合成了L ＝ 1,2,4,5-环己烷四甲酸）。此外，还检测了该化合物对肾小球肾炎治疗的生物学作用，并研究了其详细机制。首先，使用细胞计数试剂盒8（CCK-8）方法检测肾小球系膜细胞的增殖。然后，用膜联蛋白V-FITC / PI法测定细胞周期停滞和凋亡率。接下来，通过应用蛋白质印迹法检测系膜细胞中caspase-8和caspase-3的表达水平。最后，通过ELISA检测试剂盒评估释放到血浆中的炎性细胞因子。