Growth traits and genetic diversity of 23 provenances of Quercus rubra introduced from North America were analyzed in a provenance trial established with a randomized block design in Hwaseong, Gyeonggi, South Korea in 1993. Growth variables and survival at age 25 were compared with results from early stages. Height, DBH, volume and stem straightness of Q. rubra was better than those of the domestic oak (Quercus accutissima). Growth of the Dunham Island provenance from New York was the best among the 23 provenances that of the Eagle River provenance from Wisconsin was poorest. Survival rate at age 25 was on average 52%. The longitude of seed origin and growth of provenance were consistently significantly negatively correlated at all ages. Growth of coastal provenances was superior to that of the inland provenances, which were separated by the Appalachian Mountains. Genetic diversity and genetic distance among the provenances were evaluated using microsatellite markers. The allelic frequencies showed high polymorphism in 10 microsatellite loci, and 292 alleles were found. Of 10 loci, two were commonly found in the 23 provenances. The mean allelic diversity and heterozygosity observed among the provenances were similar to those from the native populations of Q. rubra in North America. Nei′s genetic distance among the 23 was estimated and showed that a clear trend between geographic and genetic distances, indicating that some provenances have high genetic diversity with superior growth performance.