Introgression of desirable traits from wild relatives plays an important role in crop improvement, as wild species have important characters such as high resistance to pests and pathogens. However, use of wild peanut relatives is challenging because almost all wild species are diploid and sexually incompatible with cultivated peanut, which is tetraploid (AABB genome type; 2n = 4x = 40). To overcome the ploidy barrier, we used 2 wild species to make a tetraploid with the same allotetraploid genome composition as cultivated peanut. Crosses were made between 2 diploid wild species, Arachis valida Krapov. and W.C. Greg. (BB genome; 2n = 2x = 20) and Arachis stenosperma Krapov. and W.C. Greg. (AA genome; 2n = 2x = 20). Cuttings from the diploid F₁ AB hybrid were treated with colchicine to induce chromosome doubling thus generating an induced allotetraploid. Chromosome counts confirmed polyploidy (AABB genome; 2n = 4x = 40). We named the new allotetraploid ValSten. Plants had well-developed fertile pollen, produced abundant seed and were sexually compatible with cultivated peanut. ValSten exhibits the same high resistance to early and late leaf spot and rust as its diploid parents. Notably, we observed morphological variations, including flower width and branch angles in the earliest generation (S₀) of allotetraploids. A SNP array was used to genotype 47 S₀ allotetraploids. The great majority of markers showed the additive allelic state from both parents (AABB). However, some loci were AAAA or BBBB, indicating homeologous recombination. ValSten provides a new, vigorous, highly fertile, disease resistant germplasm for peanut research and improvement.

来自野生近缘种的理想性状的渗入在作物改良中起着重要作用，因为野生物种具有诸如对害虫和病原体的高抗性等重要特征。但是，使用野生花生亲缘种具有挑战性，因为几乎所有野生物种都是二倍体，并且与栽培花生为四倍体（AABB基因组类型； 2 n  = 4x​​ = 40）在性上不相容。为了克服倍性障碍，我们使用2种野生物种制作了与栽培花生具有相同的四倍体基因组组成的四倍体。在2个二倍体野生物种Arachis Valida Krapov之间进行杂交。和WC Greg。（BB基因组； 2 n  = 2x = 20）和Arachis stenosperma Krapov。和WC Greg。（AA基因组; 2 n = 2x = 20）。用秋水仙碱处理来自二倍体F ₁ AB杂种的插条以诱导染色体加倍，从而产生诱导的异源四倍体。染色体计数证实了多倍性（AABB基因组； 2 n  = 4x​​ = 40）。我们将新的异源四倍体命名为ValSten。植物具有发达的可育花粉，产生了丰富的种子，并且与栽培花生具有性相容性。ValSten对二倍体亲本表现出相同的高抗早，晚叶斑病和锈病的能力。值得注意的是，我们观察到形态变异，包括最早发生于异源四倍体中的花宽和分支角（S ₀）。使用SNP阵列对47 S ₀进行基因分型异源四倍体。绝大多数标记物显示了来自两个亲本的加性等位基因状态（AABB）。但是，一些基因座是AAAA或BBBB，表明同源重组。ValSten为花生的研究和改良提供了一种新的，有活力的，高繁殖力的，抗病的种质。