Extensive transcriptomic skimming was conducted to decipher molecular, morphological, physiological, and biochemical responses in salt-tolerant (PDL-1) and salt-sensitive (L-4076) cultivars under control (0 mM NaCl) and salinity stress (120 mM NaCl) conditions at seedling stage. Morphological, physiological, and biochemical studies revealed that PDL-1 exhibited no salt injury and had higher K⁺/Na⁺ ratio, relative water content (RWC), chlorophyll, glycine betaine, and soluble sugars in leaves while lower H₂O₂ induced fluorescence signals in roots as compared to L-4076. Transcriptomic profile revealed a total of 17,433 significant differentially expressed genes (DEGs) under different treatments and cultivar combinations that include 2557 upregulated and 1533 downregulated transcripts between contrasting cultivars under salt stress. Accuracy of transcriptomic analysis was validated through quantification of 10 DEGs via quantitative real-time polymerase chain reaction (qRT-PCR). DEGs were functionally characterized by Gene Ontology (GO) analysis and assigned to various metabolic pathways using MapMan. DEGs were found to be significantly associated with phytohormone-mediated signal transduction, cellular redox homoeostasis, secondary metabolism, nitrogen metabolism, and cellular stress signaling. The present study revealed putative molecular mechanism of salinity tolerance in lentil together with identification of 5643 simple sequence repeats (SSRs) and 176,433 single nucleotide polymorphisms (SNPs) which can be utilized to enhance linkage maps density along with detection of quantitative trait loci (QTLs) associated with traits of interests. Stress-related pathways identified in this study divulged plant functioning that can be targeted to improve salinity stress tolerance in crop species.

进行了广泛的转录组撇取以破译耐盐 (PDL-1) 和盐敏感 (L-4076) 栽培品种在控制 (0 mM NaCl) 和盐度胁迫 (120 mM NaCl) 下的分子、形态、生理和生化反应苗期条件。形态、生理和生化研究表明，PDL-1 无盐害，具有较高的 K ⁺ /Na ⁺比、相对含水量 (RWC)、叶绿素、甘氨酸甜菜碱和叶片中的可溶性糖，而 H ₂ O ₂较低与 L-4076 相比，在根中诱导荧光信号。转录组学谱揭示了在不同处理和栽培品种组合下共有 17,433 个显着差异表达基因 (DEG)，其中包括在盐胁迫下对比栽培品种之间的 2557 个上调和 1533 个下调转录本。通过定量实时聚合酶链反应 (qRT-PCR) 对 10 个 DEG 进行定量，验证了转录组学分析的准确性。DEGs 通过基因本体论 (GO) 分析进行功能表征，并使用 MapMan 分配到各种代谢途径。发现 DEG 与植物激素介导的信号转导、细胞氧化还原稳态、次级代谢、氮代谢和细胞应激信号显着相关。本研究揭示了小扁豆耐盐性的推定分子机制，并鉴定了 5643 个简单序列重复 (SSR) 和 176,433 个单核苷酸多态性 (SNP)，可用于提高连锁图密度以及数量性状基因座 (QTL) 的检测与兴趣特征有关。本研究中确定的与胁迫相关的途径揭示了植物功能，可用于提高作物物种的盐胁迫耐受性。