A bacterial strain, designated 5J-6 T , was isolated from soil in Cheongnyeongpo, Republic of Korea. Cells were Gram-stain-positive, strictly aerobic, and motile rods and their catalase and oxidase activities were positive. Strain 5J-6 T grew at 10–30 °C, pH 6.0–9.0, and 0–0.8% (w/v) NaCl concentration, with optimum growth at 25 °C, pH 6.5, and 0.4% NaCl concentration. Anteiso-C 15:0 and iso-C 16:0 were detected as the predominant fatty acids and menaquinone-7 was the sole isoprenoid quinone detected. Strain 5J-6 T contained phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid as major polar lipids. The peptidoglycan belonged to the type A1γ meso -diaminopimelic acid. The G+C content of the genomic DNA calculated from the whole genomic sequence was 46.1 mol%. Phylogenetic analysis of strain 5J-6 T based on 16S rRNA gene sequences placed the isolate into a member of the genus Paenibacillus . Sequence similarity analysis of 16S rRNA gene sequences revealed that strain 5J-6 T was most closely related to Paenibacillus aceris KUDC4121 T and Paenibacillus chondroitinus DSM 5051 T with 98.76% and 98.42% similarities, respectively. Average nucleotide identity and in silico DNA–DNA hybridization values between strain 5J-6 T and the type strain of P. aceris were 83.97% and 28.60%, respectively. Based on the phylogenetic and phenotypic characteristics and genomic data, strain 5J-6 T could be considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus silvestris sp. nov. is proposed. The type strain is 5J-6 T (= KACC 21430 T = JCM 33812 T ).

中文翻译：

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类芽孢杆菌 silvestris sp。11 月，从森林土壤中分离

从大韩民国清宁浦的土壤中分离出一种菌株，命名为 5J-6 T。细胞为革兰氏染色阳性、严格需氧且能运动的杆状细胞，其过氧化氢酶和氧化酶活性为阳性。菌株 5J-6 T 在 10–30 °C、pH 6.0–9.0 和 0–0.8% (w/v) NaCl 浓度下生长，在 25 °C、pH 6.5 和 0.4% NaCl 浓度下生长最佳。Anteiso-C 15:0 和iso-C 16:0 被检测为主要脂肪酸，甲基萘醌7 是唯一检测到的异戊二烯醌。菌株 5J-6 T 含有磷脂酰乙醇胺、磷脂酰甘油和一种身份不明的磷脂作为主要的极性脂质。肽聚糖属于A1γ型内消旋-二氨基庚二酸。从全基因组序列计算的基因组 DNA 的 G+C 含量为 46.1 mol%。基于 16S rRNA 基因序列的菌株 5J-6 T 的系统发育分析将分离物置于类芽孢杆菌属的成员中。16S rRNA 基因序列的序列相似性分析表明，菌株 5J-6 T 与 Paenibacillus aceris KUDC4121 T 和 Paenibacillus chondroitinus DSM 5051 T 的相似性分别为 98.76% 和 98.42%。菌株 5J-6 T 与 P. aceris 典型菌株之间的平均核苷酸同一性和计算机 DNA-DNA 杂交值分别为 83.97% 和 28.60%。基于系统发育和表型特征以及基因组数据，可以认为菌株 5J-6 T 代表了类芽孢杆菌属的一个新种，其名称为 Paenibacillus silvestris sp。十一月 被提议。典型菌株是 5J-6 T (= KACC 21430 T = JCM 33812 T)。