Rhinanthus major and R. minor are two annual plant species that are known to hybridize in nature and in which unilateral introgression is likely to occur. Here we used double-digest restriction site-associated DNA sequencing technology (ddRAD-seq) to detect 16,932 genome-wide SNPs in R. major and R. minor individuals from 9 populations. After screening these SNP markers across both species, we selected 1106 putative loci that contain diagnostic, species-specific SNPs, which can be used for assessing and monitoring hybridization and introgression between R. major and R. minor.

中文翻译：

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使用ddRAD-seq开发了大量诊断性SNP标记，以研究大叶金枪鱼和次要R.Rananthus（Orobanchaceae）的杂交

Rhinanthus major和R. minor是两个一年生植物，已知在自然界杂交，并且可能单方面渗入。在这里，我们使用了双消化限制性位点相关的DNA测序技术（ddRAD-seq）在来自9个种群的主要和次要个体中检测了16,932个全基因组SNP 。在两个物种中筛选了这些SNP标记后，我们选择了1106个推定的基因座，这些基因座包含诊断性，物种特异性SNP，可用于评估和监测大R.和小R.的杂交和基因渗入。