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Development of a large set of diagnostic SNP markers using ddRAD-seq to study hybridization in Rhinanthus major and R. minor (Orobanchaceae)
Conservation Genetics Resources ( IF 0.9 ) Pub Date : 2021-01-03 , DOI: 10.1007/s12686-020-01181-5
Khaled Mirzaei , Renate A. Wesselingh

Rhinanthus major and R. minor are two annual plant species that are known to hybridize in nature and in which unilateral introgression is likely to occur. Here we used double-digest restriction site-associated DNA sequencing technology (ddRAD-seq) to detect 16,932 genome-wide SNPs in R. major and R. minor individuals from 9 populations. After screening these SNP markers across both species, we selected 1106 putative loci that contain diagnostic, species-specific SNPs, which can be used for assessing and monitoring hybridization and introgression between R. major and R. minor.



中文翻译:

使用ddRAD-seq开发了大量诊断性SNP标记,以研究大叶金枪鱼和次要R.Rananthus(Orobanchaceae)的杂交

Rhinanthus majorR. minor是两个一年生植物,已知在自然界杂交,并且可能单方面渗入。在这里,我们使用了双消化限制性位点相关的DNA测序技术(ddRAD-seq)在来自9个种群的主要次要个体中检测了16,932个全基因组SNP 。在两个物种中筛选了这些SNP标记后,我们选择了1106个推定的基因座,这些基因座包含诊断性,物种特异性SNP,可用于评估和监测R.R.的杂交和基因渗入。

更新日期:2021-01-03
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