Abstract

Among cereals, sorghum, a gluten-free cereal is a rich source of bioactive polyphenols and dietary antioxidants. Sorghum polyphenolic compounds were characterized and quantified in five sorghum genotypes by high performance liquid chromatography. Grain samples of five sorghum genotypes were grounded and subjected top extraction for profile of polyphenols via High Performance Liquid Chromotography. Results showed that red and brown pericarp genotypes had higher total proanthocyanidin and total phenolic content, therefore, resulting in overall high antioxidant activities. The phenolic acids that showed the higher antioxidant activity in red (SSG 59-3) and brown (G-46) genotypes were caffeic acid, taxifolin and apigeninidin. SSG 59-3 (20.55 ± 0.11^a, 45.66 ± 0.23^b, 15.34 ± 0.10^c) had higher antioxidant activity evaluated by DPPH, ABTS and FRAP assay. The concentrations of the sorghum-specific 3-deoxyanthocyanidins luteolinidin and apigeninidin were higher in red sorghum. Correlation analysis showed that antioxidant activity had a linear relationship with their polyphenols. Thus, our findings indicated that there is cumulative effect of both high antioxidant activity and phenolic compounds which may be exploited for human food to improve the nutritional value and health properties.

Graphical abstract

中文翻译：

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高粱[高粱]颗粒中酚类化合物的表征和抗氧化活性

摘要

在谷物中，高粱，无麸质谷物是生物活性多酚和饮食抗氧化剂的丰富来源。通过高效液相色谱法对五种高粱基因型中的高粱多酚化合物进行了表征和定量。将五种高粱基因型的谷物样品磨碎，并通过高效液相色谱法进行顶部提取，以测定多酚的概况。结果表明，红色和棕色果皮基因型具有较高的总原花色素和总酚含量，因此总体上具有较高的抗氧化活性。在红色（SSG 59-3）和棕色（G-46）基因型中表现出较高抗氧化活性的酚酸是咖啡酸，滑石粉和芹菜素。SSG 59-3（20.55±0.11 ^a，45.66±0.23 ^b，15.34±0.10^c）通过DPPH，ABTS和FRAP分析评估具有较高的抗氧化活性。高粱特有的3-脱氧花青素，黄体生成素和芹菜素的浓度在红色高粱中较高。相关分析表明，抗氧化剂活性与其多酚具有线性关系。因此，我们的发现表明，高抗氧化剂活性和酚类化合物都有累积作用，可用于人类食品以改善营养价值和健康特性。

图形概要