Lipids are excellent biomarkers for assessing coral stress, although staghorn coral data (Acropora cervicornis) is lacking. Lipid extraction is the most critical step in lipidomic assessments, usually performed using carcinogenic solvents. Efficient alternative using less toxic methods, such as the BUME method using butanol and methanol as extraction solvents, have not been applied to coral lipidomics evaluations. Thus, we aimed to develop a lipidomic approach to identify important coral health biomarkers by comparing different solvent mixtures in staghorn corals. Total lipid extraction was equivalent for both tested methods, but due to its efficiency in extracting polar lipids, the BUME method was chosen. It was then applied to different coral masses (0.33–1.00 g), resulting in non-significant differences concerning number of lipid classes and compounds. Therefore, this method can be successfully applied to coral assessments in a climate change context, with the added benefit of low sample masses, lessening coral sampling impacts.

脂质是用于评估珊瑚礁应力优良的生物标记物，虽然鹿角珊瑚数据（鹿角cervicornis） 是缺乏。脂质提取是脂质组学评估中最关键的步骤，通常使用致癌溶剂进行。使用毒性较小的方法的有效替代方法，例如使用丁醇和甲醇作为提取溶剂的 BUME 方法，尚未应用于珊瑚脂质组学评估。因此，我们旨在开发一种脂质组学方法，通过比较鹿角珊瑚中的不同溶剂混合物来识别重要的珊瑚健康生物标志物。两种测试方法的总脂质提取效果相同，但由于其提取极性脂质的效率，因此选择了 BUME 方法。然后将其应用于不同的珊瑚群（0.33-1.00 g），导致脂质类别和化合物数量的非显着差异。因此，这种方法可以成功地应用于气候变化背景下的珊瑚评估，