Phenanthrene (PHE) as a tricyclic polycyclic aromatic hydrocarbon is one of the common pollutants in water and sediments, which can cause reproductive toxicity to aquatic organisms. In this study, enzyme-linked immunosorbent assay (ELISA) was used to detect the vitellogenin (VTG) of loach, and then to explore the estrogenic toxicity effect of PHE on loach. The results were as follows: (1) with the increase of PHE concentrations and the extension of exposure time, the gonadosomatic index (GSI) of males decreased significantly, while it increased in female loaches. In addition, males had more obvious changes than females and were more sensitive to PHE. (2) The increase of VTG contents in serum of males were stronger than that in females. Those results reveal that PHE has estrogenic effect, which can affect the generation of VTG, thus causing damage to the gonad development of loach.

菲（PHE）是一种三环多环芳烃，是水和沉积物中的常见污染物之一，可对水生生物造成生殖毒性。本研究采用酶联免疫吸附试验（ELISA）检测泥lo中的卵黄蛋白原（VTG），进而探讨PHE对泥ach的雌激素毒性作用。结果如下：（1）随着PHE浓度的升高和接触时间的延长，雄性腺的性腺体指数（GSI）明显降低，而雌性性腺中的性腺体指数升高。此外，男性比女性有更明显的变化，并且对PHE更为敏感。（2）男性血清中VTG含量的增加要强于女性。这些结果表明，PHE具有雌激素作用，可以影响VTG的产生，