Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass. These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield. A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent. The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm² and pulse factor of 0.57. In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm² and a pulse factor of 0.93. Structural analysis of extracted polysaccharide was performed using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermal property. The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively. The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds. Furthermore, UAE and PHWE are green technologies, which can be operated at an industrial scale for PEF extraction.

超声辅助提取 (UAE) 和加压热水提取 (PHWE) 作为先进的清洁方法进行了测试，以从Phoma dimorpha菌丝体生物质中获取多糖。在富含多糖的馏分 (PEF) 产量方面，将这些方法与传统提取（热水提取，HWE）进行了比较。对每种提取方法进行了中心复合旋转设计，以研究自变量对收率的影响，并帮助选择以水为提取溶剂的收率最高的条件。PEF 的最佳提取条件为 12.02 wt%，在 75.11 W / cm ²强度下使用阿联酋直接超声处理 30 分钟时达到和脉冲因子为 0.57。在动力学曲线中，在 75.11 W / cm ²的超声强度和 0.93 的脉冲因子下，在 50°C 下获得了最高的产率（15.28 wt%）。使用傅里叶变换红外光谱、X 射线衍射、扫描电子显微镜和热性质对提取的多糖进行结构分析。PEF的水溶性指数、持水量和乳化指数分别为31.3±1.5%、138.1±3.2%和62.9±2.3%。深层发酵展示了Phoma dimorpha的巨大潜力与商业石油衍生化合物相比，生产具有生物乳化特性的多糖作为生物技术清洁剂的替代品。此外，UAE 和 PHWE 是绿色技术，可以在工业规模上用于 PEF 提取。