Due to the unique advantages comparing with traditional free enzymes and chemical catalysis, whole-cell biocatalysts have been widely used to catalyze reactions effectively, simply and environment friendly. Cell-surface display technology provides a novel and effective approach for improved whole-cell biocatalysts expressing heterologous enzymes on the cell surface. They can overcome the substrate transport limitation of the intracellular expression and provide the enzymes with enhanced properties. Among all the host surface-displaying microorganisms, yeast is ideally suitable for constructing whole cell-surface-displaying biocatalyst, because of the large cell size, the generally regarded as safe (GRAS) status, and the perfect post-translational processing of secreted proteins. Yeast cell-surface display system has been a promising and powerful method for development of novel and improved engineered biocatalysts. In this review, the characterization and principles of yeast cell-surface display and the applications of yeast cell-surface display in engineered whole-cell biocatalysts as well as the improvement of the enzyme efficiency are summarized and discussed.

由于与传统的游离酶和化学催化相比具有独特的优势，全细胞生物催化剂被广泛用于有效、简单和环境友好地催化反应。细胞表面展示技术为改进的全细胞生物催化剂在细胞表面表达异源酶提供了一种新颖有效的方法。它们可以克服细胞内表达的底物运输限制，并为酶提供增强的特性。在所有的宿主表面展示微生物中，酵母非常适合构建全细胞表面展示生物催化剂，因为它具有较大的细胞尺寸、普遍认为安全（GRAS）的状态以及对分泌蛋白的完美翻译后加工. 酵母细胞表面展示系统一直是开发新型和改进的工程生物催化剂的一种有前途和强大的方法。在这篇综述中，总结和讨论了酵母细胞表面展示的表征和原理、酵母细胞表面展示在工程化全细胞生物催化剂中的应用以及酶效率的提高。