The purpose of this study was to scan variants in coding region of Krȕppel like factor14 (KLF14) locus and assess association related to type 2 diabetes (T2D) in Iranian population. We sequenced the coding region of KLF14 to scan variants in case–sibling study (92 individuals with T2D and 92 healthy older siblings). To confirm, we analyzed rs76603546 association with T2D in a larger unrelated case–control study by PCR–RFLP (475 cases and 512 controls). We analyzed the association of rs76603546 with HbA_1C, BMI, fat mass, waist circumference, fasting glucose, cholesterol and HOMA-IR (Homeostatic Model Assessment for Insulin Resistance) using one-way ANOVA analysis. Also, association of genotypes with T2D adjusted for confounding variables was analyzed using logistic regression. HaploReg v 4.1 was used to predict rs76603546 possible function. Sequencing results analysis revealed the association of C allele of rs76603546, synonymous variant C>T, [OR 2.10 (1.38–3.20), P value < 0.001] and CC genotype of rs76603546 [OR 4.3 (1.79–10.23), P value = 0.001] with susceptibility to T2D. PCR-Restriction Fragment Length Polymorphism (RFLP) results analysis confirmed the association of rs76603546 with T2D [C allele, OR 1.91 (1.59–2.29), P value = 0.002, CC genotype, OR 3.27 (2.26–4.73), P value = 0.002 and TC genotype, OR 1.74 (1.31–2.31), P value = 0.001]. The CC genotype of rs76603546 is associated with HbA_1C level (P value < 0.001) and BMI (P value = 0.02). After adjustment with confounding variables, we observed association of CC genotype with T2D [OR 2.542 (1.25–3.77), P value = 0.03]. Among over 220 SNPs, rs76603546 was associated with T2D, HbA_1C and BMI in our study.

这项研究的目的是扫描Krȕppel样因子14（KLF14）基因座编码区的变异，并评估与伊朗人群中2型糖尿病（T2D）相关的关联。在案例研究中，我们对KLF14的编码区进行了测序，以扫描变体（92名患有T2D的个体和92名健康的老年同胞）。为了证实这一点，我们通过PCR-RFLP（475例和512例对照）在一项更大的无关病例对照研究中分析了rs76603546与T2D的关联。我们分析了rs76603546与HbA _1C的关联，BMI，脂肪量，腰围，空腹血糖，胆固醇和HOMA-IR（胰岛素抵抗稳态模型评估），采用单向ANOVA分析。此外，使用逻辑回归分析了基因型与针对混杂变量调整的T2D的关联。HaploReg v 4.1用于预测rs76603546的可能功能。测序结果分析显示，rs76603546的C等位基因，同义变体C> T，[OR 2.10（1.38–3.20），P值<0.001]和CC基因型与rs76603546的关联[OR 4.3（1.79–10.23），P值= 0.001对T2D敏感。PCR-限制性片段长度多态性（RFLP）结果分析证实了rs76603546与T2D相关联[C等位基因，或1.91（1.59–2.29），P值= 0.002，CC基因型，OR 3.27（2.26-4.73），P值= 0.002，TC基因型，OR 1.74（1.31-2.31），P值= 0.001]。rs76603546的CC基因型与HbA _1C水平（P值<0.001）和BMI（P值= 0.02）相关。用混杂变量进行调整后，我们观察到CC基因型与T2D的相关性[OR 2.542（1.25–3.77），P值=  0.03 ]。在我们的研究中，在220多个SNP中，rs76603546与T2D，HbA _1C和BMI相关。