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Evaluation of interaction among indigenous rhizobacteria and Vigna unguiculata on remediation of metal-containing abandoned magnesite mine tailing
Archives of Microbiology ( IF 2.3 ) Pub Date : 2021-01-03 , DOI: 10.1007/s00203-020-02115-3
Mathiyazhagan Narayanan , Muthusamy Ranganathan , Gajendiran Kandasamy , Suresh Kumarasamy

Abandoned magnesite mine heap causing pollution to nearby farmland and water reservoir. Thus the intention of this research was to screening metal mobilizing and absorbing bacteria from the rhizosphere section of V. unguiculata from farmland nearby to magnesite mine. Further, studied their stimulus effect on growth, biomass, and phytoextraction prospective of V unguiculata in mine tailing. The results of the physicochemical properties of mine tailing shows that four metals (Pb, Mn, Cd, and Zn) were crossing the permissible limit. Out of 27 isolates, 2 isolates (MMS15 and MMS17) were identified with maximum metal tolerance for up to 700 mg L-1 (MIC) and metal mobilization (Pb 5.5 and 5.87, Mn 6.6 and 4.88, Cd 1.99 and 2.59, and Zn 6.55 and 6.94 mg kg-1) and biosorption efficiency as Pb 3.74 and 3.74, Mn 4.9 and 4.7, Cd 2.41 and 3.96, and Zn 4.3 and 4.9 mg g-1. These two strains were identified as members of B. cereus and Kosakonia sp. using 16S rRNA technique and labelled strains NDRMN001 and MGR1, respectively. The Kosakonia sp. MGR1 effectively fixes the nitrogen in the rate of 81.94% and B. cereus NDRMN001 solubilizes 69.98 ± 2.31 mg L-1 of soluble phosphate. The experimental group's study results show that the group C (Kosakonia sp. MGR1 and B. cereus NDRMN001) has effectively stimulate the growth, biomass, and phytoextraction potential of V. unguiculata. The results conclude that the optimistic interaction between these two bacteria could be more significant to minimize the metal pollution in magnesite mine tailing.

中文翻译:

土著根际细菌与豇豆相互作用对含金属废弃菱镁矿尾矿修复的评价

废弃的菱镁矿堆对附近农田和水库造成污染。因此,本研究的目的是从附近农田到菱镁矿的 V. unguiculata 根际部分筛选金属动员和吸收细菌。进一步研究了它们对尾矿中V unguiculata 生长、生物量和植物提取前景的刺激作用。尾矿理化性质的结果表明,四种金属(Pb、Mn、Cd 和 Zn)超过了允许限值。在 27 个分离株中,确定了 2 个分离株(MMS15 和 MMS17)对高达 700 mg L-1(MIC)和金属迁移(Pb 5.5 和 5.87,Mn 6.6 和 4.88,Cd 1.99 和 2.59,以及 Zn 6.55 和 6.94 mg kg-1),生物吸附效率为 Pb 3.74 和 3.74、Mn 4.9 和 4.7、Cd 2.41 和 3.96 以及 Zn 4。3 和 4.9 毫克 g-1。这两个菌株被鉴定为蜡状芽孢杆菌和 Kosakonia sp. 的成员。分别使用 16S rRNA 技术和标记菌株 NDRMN001 和 MGR1。Kosakonia sp。MGR1 有效固氮率为 81.94%,蜡状芽孢杆菌 NDRMN001 可溶解 69.98 ± 2.31 mg L-1 的可溶性磷酸盐。实验组的研究结果表明,C组(Kosakonia sp. MGR1和B. cereus NDRMN001)有效地刺激了V. unguiculata的生长、生物量和植物提取潜力。结果得出结论,这两种细菌之间的乐观相互作用对于最大限度地减少菱镁矿尾矿中的金属污染可能更重要。分别。Kosakonia sp。MGR1 有效固氮率为 81.94%,蜡状芽孢杆菌 NDRMN001 可溶解 69.98 ± 2.31 mg L-1 的可溶性磷酸盐。实验组的研究结果表明,C组(Kosakonia sp. MGR1和B. cereus NDRMN001)有效地刺激了V. unguiculata的生长、生物量和植物提取潜力。结果得出结论,这两种细菌之间的乐观相互作用对于最大限度地减少菱镁矿尾矿中的金属污染可能更重要。分别。Kosakonia sp。MGR1 有效固氮率为 81.94%,蜡状芽孢杆菌 NDRMN001 可溶解 69.98 ± 2.31 mg L-1 的可溶性磷酸盐。实验组的研究结果表明,C组(Kosakonia sp. MGR1和B. cereus NDRMN001)有效地刺激了V. unguiculata的生长、生物量和植物提取潜力。结果得出结论,这两种细菌之间的乐观相互作用对于最大限度地减少菱镁矿尾矿中的金属污染可能更重要。V. unguiculata 的植物提取潜力。结果得出结论,这两种细菌之间的乐观相互作用对于最大限度地减少菱镁矿尾矿中的金属污染可能更重要。V. unguiculata 的植物提取潜力。结果得出结论,这两种细菌之间的乐观相互作用对于最大限度地减少菱镁矿尾矿中的金属污染可能更重要。
更新日期:2021-01-03
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