Diabetes and its related complications are responsible for severe morbidity and deaths round the globe. Diabetic foot infections (DFIs) are severe and dreadful complication of diabetes mellitus. DFI/DFUs are colonized with numerous pathogenic, multi-drug-resistant microorganisms and progression in colonization of these microorganism leads to biofilm establishment making treatment challenging. There is an indispensable requirement for alternative approaches to combat this threat. In the present assignment, titanium oxide nanoparticles (TiO₂-NPs) were fabricated from the leaf extract of Ochradenus arabicus and inhibition of biofilm and related functions was examined against multi-drug-resistant strains of P. aeruginosa and methicillin-resistant S. aureus (MRSA) isolated from foot ulcers. Bio-fabricated TiO₂-NPs reduced biofilm formation in the selected strains by 22–70% at tested sub-MICs. Significant reduction in alginate and severely impaired motility of P. aeruginosa strains was also observed. Key functions such as cell surface hydrophobicity and EPS production that contribute in the attachment and maintenance of biofilm, respectively, were also inhibited considerably in test strains at sub-MICs. Statistically significant reduction (51–63%) in the preformed biofilms of all strains was also recorded. Interaction of bacterial cell and TiO₂-NPs resulted in increased levels of ROS, and this could be the plausible the reason for the biofilm inhibitory action of NPs. These NPs could be exploited in the current treatment strategies to combat the threat of biofilm-based drug-resistant diabetic foot infections and facilitate wound healing.

糖尿病及其相关并发症导致全球范围内的严重发病和死亡。糖尿病足感染（DFI）是糖尿病的严重且可怕的并发症。DFI / DFU被许多病原性，多药耐药性微生物定殖，这些微生物定殖的进展导致生物膜的形成，使治疗面临挑战。对抗这种威胁的替代方法是必不可少的。另外，在本分配，氧化钛纳米颗粒（氧化钛₂ -nps）从的叶提取物被制造Ochradenus arabicus和生物膜和相关功能抑制对的多药耐药菌株检测铜绿假单胞菌和耐甲氧西林从足溃疡中分离出金黄色葡萄球菌（MRSA）。在测试的亚MIC下，生物制备的TiO ₂ -NP可将所选菌株中的生物膜形成减少22–70％。还观察到铜绿假单胞菌菌株的藻酸盐显着降低和运动能力严重受损。亚MICs的测试菌株也分别抑制了关键功能，例如分别有助于生物膜附着和维持的细胞表面疏水性和EPS产生。还记录了所有菌株预先形成的生物膜的统计学显着减少（51-63％）。细菌细胞与TiO _2的相互作用-NPs导致ROS水平升高，这可能是NPs生物膜抑制作用的原因。在目前的治疗策略中可以利用这些NP来对抗基于生物膜的抗药性糖尿病足感染的威胁并促进伤口愈合。