Introduction

Psychoda sp. is often collected from patchy habitats such as sewers, drains and decomposing organic matters. The discovery of Psychoda sp. in forensic studies indicated that it might have noteworthy value in assisting death investigations.

Purpose

This study reports on the first finding of Psychoda larvae collected from decomposing rabbit carcasses placed in Cameron Highlands, Pahang, Malaysia.

Methods

The larvae were first observed on rabbit carcasses and were collected using tweezers and carefully preserved in 70% ethanol. They were subsequently mounted on microscopy slides using Hoyer’s medium and identified as Psychoda sp. morphologically. The identification was also confirmed through a DNA barcoding analysis.

Results

Psychoda sp. larvae were collected on day-10 post-mortem where the rabbit carcasses were at the advanced decay stage of decomposition. The cytochrome c oxidase I (COI) gene sequences of the larvae had 90% similarity with the Psychoda spp. in the database.

Conclusion

The finding of these larvae on carrion may provide additional valuable insights into forensic entomology and may assist in death investigations.

中文翻译：

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排水蝇幼虫对兔子尸体的殖民化，Psychoda sp。（双翅目：Psychodidae）：第一份报告

简介

心理达sp. 通常从不规则的栖息地收集，如下水道、排水沟和分解有机物。Psychoda sp.的发现。法医研究表明，它在协助死亡调查方面可能具有显着的价值。

目的

这项研究报告了从放置在马来西亚彭亨金马伦高原的分解兔子尸体中收集到的Psychoda幼虫的第一个发现。

方法

幼虫首先在兔尸体上观察，并用镊子收集并小心保存在 70% 乙醇中。随后使用霍耶氏培养基将它们安装在显微镜载玻片上，并鉴定为Psychoda sp。形态上。鉴定也通过DNA条形码分析得到证实。

结果

心理达sp. 在死后第 10 天收集幼虫，此时兔子尸体处于分解的晚期腐烂阶段。幼虫的细胞色素 c 氧化酶 I (COI) 基因序列与Psychoda spp具有 90% 的相似性。在数据库中。

结论

在腐肉上发现这些幼虫可能会为法医昆虫学提供更多有价值的见解，并可能有助于死亡调查。