Purpose

Surra is a zoonotic disease caused by Trypanosoma evansi (Trypanozoon), a salivary trypanosome native to Africa which affects a wide range of mammals worldwide and causes mortality and significant economic loss. The present study was devoted to the molecular characterization of T. evansi derived from naturally infected dromedary camels in Algeria.

Methods

A total of 148 blood samples were collected from mixed age camels living in one of four geographic regions (Ouargla, El Oued, Biskra and Ghardaia) of Algeria. Samples underwent PCR amplification and sequencing of the internal transcribed spacer 1 (ITS1) complete sequence.

Results

DNA of Trypanosoma spp. was found in 19 camels (12.84%). Trypanosoma spp. molecular positivity was not affected by sex (p = 0.50), age (p = 0.08), or geographic location (p = 0.12). Based on multiple sequence alignment of the obtained DNA sequences with representative T. evansi ITS1 sequences available globally, the Algerian sequences were grouped within four different haplotypes including two which were original.

Conclusion

Results of this study provide preliminary data on which future studies of genetic diversity and molecular epidemiology of T. evansi can be based.

中文翻译：

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在来自阿尔及利亚的单峰骆驼中鉴定出新的伊氏锥虫单倍型

目的

Surra 是一种由伊万斯锥虫（Trypanozoon）引起的人畜共患疾病，这是一种原产于非洲的唾液锥虫，它影响全世界范围内的多种哺乳动物，并导致死亡和重大经济损失。本研究致力于从阿尔及利亚自然感染的单峰骆驼中提取的T. evansi的分子特征。

方法

从生活在阿尔及利亚四个地理区域（瓦尔格拉、埃尔乌德、比斯克拉和加尔达亚）之一的混龄骆驼中收集了总共 148 份血液样本。样品经历了内部转录间隔区 1 (ITS1) 完整序列的 PCR 扩增和测序。

结果

锥虫的DNA 。在 19 头骆驼 (12.84%) 中发现。锥虫属 分子阳性不受性别 ( p  = 0.50)、年龄 ( p  = 0.08) 或地理位置 ( p  = 0.12) 的影响。基于获得的 DNA 序列与全球可用的代表性T. evansi ITS1序列的多重序列比对，阿尔及利亚序列分为四种不同的单倍型，包括两种原始单倍型。

结论

这项研究的结果提供了初步数据，可以作为未来对T. evansi遗传多样性和分子流行病学研究的基础。