Metarhizium species are considered one of the most outstanding powerful biological control agents that have been commercialized as biopesticides against various agricultural pests. Fungal stability with successive in vitro cultivation is a desirable trait for a large-scale production of fungal biopesticide. The new Egyptian strain Metarhizium anisopliae AUMC 3262 exhibited auspicious results when compared to Metarhizium brunneum ARSEF 4556 and M. brunneum V275 based on the variations of fungal characteristics, and essential quality control parameters (radial growth rate, conidial yield, viability, and virulence) after repeated in vitro subculturing. Changes in morphological characteristics were noted at both AUMC 3262 and ARSEF 4556. Following the 5th subculture, decreased conidial yield was noted, though radial growth remained stable, confirming that there is a non-positive correlation between conidial yield and radial growth rate for these species. In contrast, V275 showed a high morphological stability, conidial yield, and radial growth rate after repeated subculture. The three tested strains manifested high viability up to 100% and displayed the same pattern of Pr1 production. A slight variation was recorded in the median lethal time (LT50) values against the great wax moth, Galleria mellonella (L.), larvae between different subcultures of the tested Metarhizium strains. The new Egyptian strain AUMC 3262 showed a high stability with a slight difference in some parameters after the successive subculture compared to both ARSEF4556 and V275.

中文翻译：

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之间的真菌稳定性的对比研究绿僵连续传代培养后的菌株

变种被认为是最杰出的强大生物防治剂之一，已作为杀虫剂被商业化用于对抗各种农业害虫。具有连续体外培养的真菌稳定性是大规模生产真菌生物农药的理想特性。基于真菌特性和基本质量控制参数（辐射生长速率，分生孢子产量，生存力和毒力）的变化，新的埃及菌株Metanhizium anisopliae AUMC 3262与布鲁氏梅氏杆菌ARSEF 4556和布鲁氏莫尔氏菌V275相比显示出吉祥的结果。重复体外传代。在AUMC 3262和ARSEF 4556上都注意到形态特征的变化。第5次传代培养后，虽然径向生长保持稳定，但分生孢子的产量却下降了，证实这些物种的分生孢子产量和径向生长率之间没有正相关。相反，在重复传代培养后，V275表现出很高的形态稳定性，分生孢子产率和径向生长速率。这三个测试菌株表现出高达100％的高生存力，并显示出相同的Pr1产生模式。记录的不同致病菌株间不同传代的大蜡蛾，Galleria mellonella（L.）幼虫的致死时间（LT50）值有轻微变化。与ARSEF4556和V275相比，新的埃及菌株AUMC 3262在连续传代培养后显示出高稳定性，某些参数略有差异。重复传代培养后，V275表现出很高的形态稳定性，分生孢子产率和径向生长速率。这三个测试菌株表现出高达100％的高生存力，并显示出相同的Pr1产生模式。记录的不同致病菌株间不同传代的大蜡蛾，Galleria mellonella（L.）幼虫的致死时间（LT50）值有轻微变化。与ARSEF4556和V275相比，新的埃及菌株AUMC 3262在连续传代培养后显示出高稳定性，某些参数略有差异。重复传代培养后，V275表现出很高的形态稳定性，分生孢子产率和径向生长速率。这三个测试菌株表现出高达100％的高生存力，并显示出相同的Pr1产生模式。记录的不同致病菌株间不同传代的大蜡蛾，Galleria mellonella（L.）幼虫的致死时间（LT50）值有轻微变化。与ARSEF4556和V275相比，新的埃及菌株AUMC 3262在连续传代培养后显示出高稳定性，某些参数略有差异。记录的不同致病菌株间不同传代的大蜡蛾，Galleria mellonella（L.）幼虫的致死时间（LT50）值有轻微变化。与ARSEF4556和V275相比，新的埃及菌株AUMC 3262在连续传代培养后显示出高稳定性，某些参数略有差异。记录的不同致病菌株间不同传代的大蜡蛾，Galleria mellonella（L.）幼虫的致死时间（LT50）值有轻微变化。与ARSEF4556和V275相比，新的埃及菌株AUMC 3262在连续传代培养后显示出高稳定性，某些参数略有差异。