Transcriptome analysis of crown gall in radish (Raphanus sativus L.) inbred lines,Annals of Applied Biology

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Transcriptome analysis of crown gall in radish (Raphanus sativus L.) inbred lines
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2021-01-02 , DOI: 10.1111/aab.12669
A.A. Tkachenko _{1,

2} , M.S. Gancheva _{1,

3} , V.E. Tvorogova ₁ , L.G. Danilov ₁ , A.V. Predeus ₄ , I.E. Dodueva ₁ , L.A. Lutova ₁

Affiliation

Studying aberrations of normal developmental processes like plant tumour initiation provides a great opportunity for understanding of systemic control of plant cells differentiation. Most well‐studied example of plant tumour is the crown gall caused by Agrobacterium tumefaciens; much less studied are spontaneous tumours developing in plants with certain genotype, regardless of agrobacterial infection. In the present study, we used RNA‐seq to analyse the changes in gene expression at the early stages of development of A. tumefaciens‐induced tumours in radish seedlings. In our studies we used two closely related radish inbred lines contrasting in their ability to form spontaneous tumours. RNA‐seq and qPCR analysis showed significant changes in the expression levels for numerous genes involved in different metabolic and developmental processes, and Gene Ontology analysis indicated many biological processes probably involved in the crown gall development in radish. For example, a unique result of our research is upregulation of genes related to photomorphogenesis and downregulation of senescence‐associated genes in the young crown gall tumours. Differences in gene expression between the analysed radish lines included upregulation of pathways related to chromatin remodelling and protein targeting to chloroplast in the tumorous line along with downregulation of pathways of chaperone‐mediated complex assembly and anthocyanin biosynthesis.

中文翻译：

萝卜近交系冠gall的转录组分析

研究正常发育过程的像差，例如植物肿瘤的萌发，为理解植物细胞分化的系统控制提供了很大的机会。关于植物肿瘤的最充分研究的例子是根癌农杆菌引起的冠gall病。不论农杆菌感染如何，在具有某些基因型的植物中发生的自发性肿瘤研究较少。在本研究中，我们使用RNA序列分析了根癌农杆菌发育早期基因表达的变化。萝卜幼苗中诱发的肿瘤。在我们的研究中，我们使用了两个紧密相关的萝卜近交系，形成自发肿瘤的能力形成了鲜明的对比。RNA-seq和qPCR分析显示了涉及不同代谢和发育过程的许多基因的表达水平发生了显着变化，而基因本体分析表明许多可能与萝卜冠in发育有关的生物学过程。例如，我们研究的独特结果是年轻冠状胆囊癌中与光形态发生相关的基因上调和衰老相关基因的下调。

更新日期：2021-01-02

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