Xylitol, a sugar substitute, presents considerable amount of benefits for both product applications and consumer health, which make it a high value product for various industries. The production of xylitol through yeast fermentation is an alternative that suits sustainable development, as it can use agro-industrial byproducts, and might come to reach a lower production price than the current method in use once its technology is further developed. While there is extensive research in the process up to the synthesis of xylitol, the purification process to attain the final product is still not well consolidated. In the present work, tests were performed to assess the capacity of purification of xylitol containing fermented media by activated carbon in fixed bed columns. Response surface methodology with pulse input was utilized to investigate how the process responses vary when temperature, superficial velocity and injection volume are altered, and to define which conditions maximize productivity and minimizes xylitol retention while maintaining satisfactory levels of clarification. The best condition found (T = 70 °C, v_s = 1.5 cm/min, V_i = 3 mL) was then applied to a new purification test with continuous feeding of fermented media. The process presented as a potential alternative for biotechnological xylitol purification step with efficacy in separating proteins (PF_protein = 18.0), colored compounds (reducing more than 99% of absorbance at 420 nm and 560 nm) and ethanol (PF_ethanol = 20.4) with a productivity of 36.8 g L⁻¹ h⁻¹ and RC_xylitol = 31.1%.

木糖醇（一种糖的替代品）在产品应用和消费者健康方面都具有可观的益处，这使其成为各个行业的高价值产品。通过酵母发酵生产木糖醇是适合可持续发展的另一种选择，因为它可以利用农业工业副产品，并且一旦其技术得到进一步发展，其价格可能会低于目前使用的方法。尽管在合成木糖醇的过程中进行了广泛的研究，但获得最终产物的纯化过程仍未得到很好的巩固。在目前的工作中，进行了测试以评估通过固定床色谱柱中的活性炭对木糖醇发酵培养基的纯化能力。利用脉冲输入的响应面方法，研究了温度，表观速度和进样量变化时过程响应如何变化，并定义了哪些条件可以最大程度地提高生产率和最小化木糖醇保留，同时保持令人满意的澄清度。找到最佳条件（T = 70°C，v_然后将s = 1.5 cm / min，_Vi = 3 mL）用于新的提纯试验，同时连续加入发酵培养基。该工艺可作为生物技术木糖醇纯化步骤的潜在替代方法，具有分离蛋白质（PF_蛋白= 18.0），有色化合物（在420 nm和560 nm处吸光度降低99％以上）和乙醇（PF_乙醇= 20.4）的功效。生产率为36.8 g L ^-1 h ^-1，RC_木糖醇= 31.1％。