Both the benefits of bacterial quorum sensing (QS) and cross-feeding for bio-reactor performance in wastewater treatment have been recently reported. As the social traits of microbial communities, how bacterial QS regulating bacterial trade-off by cross-feeding remains unclear. Here, we find diffusion signal factor (DSF), a kind of QS molecules, can bridge bacterial interactions through regulating public goods (extracellular polymeric substances (EPS), amino acids) for metabolic cross-feedings. It showed that exogenous DSF-addition leads to change of public goods level and community structure dynamics in the anammox consortia. Approaches involving meta-omics clarified that anammox and a Lautropia-affiliated species in the phylum Proteobacteria can supply costly public goods for DSF-Secretor species via secondary messenger c-di-GMP regulator (Clp) after sensing DSF. Meanwhile, DSF-Secretor species help anammox bacteria scavenge extracellular detritus, which creates a more suitable environment for the anammox species, enhances the anammox activity, and improves the nitrogen removal rate of anammox reactor. The trade-off induces discrepant metabolic loads of different microbial clusters, which were responsible for the community succession. It illustrated the potential to artificially alleviate metabolic loads for certain bacteria. Deciphering microbial interactions via QS not only provides insights for understanding the social behavior of microbial community, but also creates new thought for enhancing treatment performance through regulating bacterial social traits via quorum sensing-mediated public goods.

最近已经报道了细菌群体感应（QS）和交叉进料对生物反应器在废水处理中的性能的好处。作为微生物群落的社会特征，尚不清楚细菌QS如何通过交叉喂养来调节细菌的权衡。在这里，我们发现扩散信号因子（DSF）是一种QS分子，可以通过调节公共交叉代谢的公共物品（细胞外聚合物（EPS），氨基酸）来桥接细菌相互作用。结果表明，外源性DSF的添加导致厌氧菌财团中公共产品水平和社区结构动态的变化。涉及组学组学的方法阐明，变形菌门中的厌氧氨氧化菌和与Lautropia相关的物种可以在感测到DSF之后通过次级信使c-di-GMP调节剂（Clp）为DSF-Secretor物种提供昂贵的公共物品。同时，DSF-Secretor菌种帮助厌氧菌清除细胞外碎屑，为厌氧菌创造了更合适的环境，提高了厌氧菌的活性，提高了厌氧菌反应器的脱氮率。权衡取舍会导致不同微生物簇的代谢负荷不同，这些微生物簇负责群落的演替。它说明了人为减轻某些细菌的代谢负荷的潜力。通过QS来了解微生物的相互作用，不仅为了解微生物群落的社会行为提供了见解，