Several vitrification protocols have been established for porcine oocytes so as to facilitate gene banking of female germplasm. Although live piglets have been successfully produced from pig oocytes vitrified at the germinal vesicle (GV) stage, the competence of vitrified oocytes to develop into the blastocyst stage is greatly compromised following cryopreservation. The focus of this review is to elucidate the impact of cryopreservation on the redox balance of pig oocytes, making special reference to the relevance of non-enzymatic and enzymatic antioxidant defences. Besides, the regulation of gene expression in response to oxidative stress is also considered. Finally, we discuss the effects of supplementing maturation and vitrification media with the exogenous non-enzymatic antioxidants that have hitherto yielded the most relevant results.

已经建立了几种用于猪卵母细胞的玻璃化方案，以促进女性种质的基因库。尽管已经从在生小泡（GV）阶段进行了玻璃化的猪卵母细胞成功地生产了活小猪，但是冷冻保存后，玻璃化的卵母细胞发展为胚泡期的能力受到了很大的损害。这篇综述的重点是阐明冷冻保存对猪卵母细胞氧化还原平衡的影响，特别提到了非酶和酶抗氧化剂防御的相关性。此外，还考虑了响应于氧化应激的基因表达的调节。最后，我们讨论了迄今为止已产生最相关结果的外源非酶抗氧化剂对成熟和玻璃化培养基的补充作用。