This study was designed to evaluate the physicochemical properties of phage P22 in different pH and antibiotic levels as measured by growth kinetics, phage adsorption, and lytic activity. P22 was susceptible to acidic pHs and stable above pH 4. The latent period of P22 was 45 min and burst size was 34 phages/cell. The adsorption ability of phage to Salmonella Typhimurium was varied depending on the multiplicity of infections (MOIs). The latent period was reduced to 6.84, 4.02, and 1.72 h, respectively, on the levels of the host at 10⁴, 10⁶, and 10⁸ CFU/ml. No significant differences in adsorption were observed between pH 4 and pH 7, but the lytic activities were significantly enhanced at the presence of ceftriaxone (CEA) and ciprofloxacin (CIP) at pH 7. Therefore, the phages combined with antibiotics can be a promising therapeutic tool to control antibiotic-resistant bacteria. This results provide a better understanding of host-phages interactions in different environmental conditions.

本研究旨在评估在不同pH和抗生素水平下噬菌体P22的理化性质，通过生长动力学，噬菌体吸附和裂解活性进行测定。P22对酸性pH敏感，在pH 4以上稳定。P22的潜伏期为45分钟，破裂大小为34个噬菌体/细胞。噬菌体对鼠伤寒沙门氏菌的吸附能力随感染的复数（MOIs）而变化。潜伏期为10降低到6.84，4.02，和1.72小时，分别在主机的电平⁴，10 ⁶和10 ⁸ CFU /毫升。在pH 4和pH 7之间没有观察到明显的吸附差异，但是在pH 7的情况下，在存在头孢曲松（CEA）和环丙沙星（CIP）的情况下，裂解活性显着提高。控制抗药性细菌的工具。该结果提供了对不同环境条件下宿主-噬菌体相互作用的更好理解。