The study aims to determine the impact of Cd (20 μM) and Zn (100 μM) in the presence or absence of Si (2 mM) on plant development and fibre differentiation in young stem of hemp (Cannabis sativa L.). Gene expression and proteome involved in fibre development were analyzed. Both elements reduced the diameter of primary bast fibres. Cadmium negatively affected cellulose and lignin biosynthesis and decreased substitution of xylan in fibres, while Zn had an opposite impact on cellulose metabolism. Only a minor proportion of proteins were affected by both Cd and Zn, suggesting that the two heavy metals have a quite different impact on protein regulation in C. sativa. Si had a specific impact on some proteins observed in CdSi treatment comparatively to plants exposed to Cd in the absence of Si: 55 % of those proteins (10 among 18) were specifically regulated by this treatment and remained unaffected by Cd or Si applied alone. Six proteins were significantly regulated in ZnSi-exposed plants comparatively to Zn-treated ones and none of them was specifically regulated by Si in the absence of Zn. Diameter of bast fibre increased in response to Si in Cd-treated plants. This confirms that the presence of protecting Si confers a specific physiological status in relation to cell wall differentiation to heavy metal-treated plants.

该研究旨在确定存在或不存在Si（2 mM）时Cd（20μM）和Zn（100μM）对大麻（Cannabis sativa L.）幼茎中植物发育和纤维分化的影响。分析了参与纤维发育的基因表达和蛋白质组。两种元素均减小了初级韧皮纤维的直径。镉对纤维素和木质素的生物合成有负面影响，并减少了木聚糖在纤维中的取代，而锌对纤维素的代谢有相反的影响。Cd和Zn都影响蛋白质的比例很小，这表明两种重金属对紫花苜蓿蛋白质调控的影响大不相同。。与在不存在Si的情况下暴露于Cd的植物相比，Si对在CdSi处理中观察到的某些蛋白质具有特定的影响：这些蛋白质中有55％（18种中的10种）受此处理特异调节，并且不受单独施用Cd或Si的影响。与锌处理过的植物相比，ZnZn处理过的植物中有6种蛋白质受到显着调节，并且在没有Zn的情况下，它们都没有被Si特异性调节。在Cd处理过的植物中，韧皮纤维的直径随Si的增加而增加。这证实了保护性Si的存在赋予了与细胞壁向重金属处理的植物分化有关的特定生理状态。