Journal of Fluorescence ( IF 2.6 ) Pub Date : 2021-01-02 , DOI: 10.1007/s10895-020-02660-6 Mahdi Mirzababaei 1 , Kambiz Larijani 1 , Hamid Hashemi-Moghaddam 2 , Zohreh Mirjafary 1 , Hamid Madanchi 3, 4
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Chemotherapy using drug delivery systems (DDS) can target cancer cells selectively and without affecting normal cells. In this paper, NL2 peptide as a tumor targeted peptide was bonded on the surface of poly 3,4-Dihydroxy-L-phenylalanine (Poly L-DOPA) graphene quantum dots (GQD), which was imprinted by Doxorubicin (DOX). The synthesized nanocomposite was characterized by Fourier-transform infrared spectroscopy (FTIR) and particle size was determined by dynamic light scattering (DLS) and Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). DOX release from synthesized nano-composite was investigated spectrophotometrically. Also, the toxicity and selectivity of NL2-GQD-NC on SK-BR-3 cell line were evaluated. FTIR and DLS experiment confirm the successful synthesis of Poly L-DOPA coated graphene quantum dots and their uniform particles. In vitro studies have shown that NL2-GQD-NC attached more to SK-BR-3 cells than NL2-free nanocomposites (GQD-NC). After attaching the cells could be imaged due to the presence of GQD particles and DOX release was accomplished in the tumor cells.
Graphical Abstract
中文翻译：
聚 L-DOPA 涂层石墨烯量子点上结合的 NL2 肽的体外靶向
使用药物输送系统（DDS）的化疗可以选择性地靶向癌细胞，而不影响正常细胞。本文将NL2肽作为肿瘤靶向肽键合在聚3,4-二羟基-L-苯丙氨酸（Poly L-DOPA）石墨烯量子点（GQD）表面，并用阿霉素（DOX）印迹。通过傅里叶变换红外光谱（FTIR）对合成的纳米复合材料进行表征，并通过动态光散射（DLS）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）测定粒径。通过分光光度法研究了合成纳米复合材料中 DOX 的释放。此外，还评估了 NL2-GQD-NC 对 SK-BR-3 细胞系的毒性和选择性。 FTIR和DLS实验证实了聚L-DOPA涂覆的石墨烯量子点及其均匀颗粒的成功合成。体外研究表明，NL2-GQD-NC 比不含 NL2 的纳米复合材料 (GQD-NC) 更多地附着在 SK-BR-3 细胞上。附着后，由于 GQD 颗粒的存在，可以对细胞进行成像，并且肿瘤细胞中完成了 DOX 的释放。
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