The emergence of COVID-19 disease due to SARS-CoV-2 at the end of 2019 was rapidly associated with the isolation of the strain from co-culture onto VERO cells. These isolations quickly made it possible to carry out the first tests for antiviral agents’ susceptibility and drug repurposing. However, it seems important to make an inventory of all the cells that can support the growth of this virus and evaluate possible differences between isolates. In the present work, we tested 4 strains of SARS-CoV-2 locally isolated on a panel of 34 cell lines present in our laboratory and commonly used for the isolation of human pathogenic microorganism. After inoculation, cells were observed for cytopathic effects and quantitative real-time polymerase reaction was used to measure the virus replication on the cells. We were able to obtain growth on 7 cell lines, 6 simian, and the human Caco-2. The cytopathogenic effects are variable, ranging from lysis of the cell monolayer in 48–72 h to no cytopathic effect in spite of intense multiplication, as in Caco-2 cells. Interestingly, effect and multiplication varied widely according to the strain tested. In this paper, we explored the species specificity and tissue tropism of SARS-CoV-2 in vitro on a panel of cells available in our laboratory and identified human and animal cell lines susceptible to support SARS-CoV-2 replication. Our work highlights the importance of testing multiple strains when testing antiviral molecules and performing patho-physiological analyzes.

2019 年底由于 SARS-CoV-2 引起的 COVID-19 疾病的出现与该菌株从共培养到 VERO 细胞上的分离迅速相关。这些分离很快使进行抗病毒药物敏感性和药物再利用的第一次测试成为可能。然而，对所有可以支持这种病毒生长的细胞进行清点并评估分离株之间可能存在的差异似乎很重要。在目前的工作中，我们测试了在我们实验室存在的一组 34 个细胞系中局部分离的 4 株 SARS-CoV-2，这些细胞系通常用于分离人类病原微生物。接种后，观察细胞的致细胞病变效应，并使用定量实时聚合酶反应测量病毒在细胞上的复制。我们能够在 7 个细胞系上获得生长，6猿猴和人类Caco-2。致细胞病变的影响是可变的，从 48-72 小时内细胞单层的裂解到尽管有强烈的增殖但没有致细胞病变的影响，如在 Caco-2 细胞中。有趣的是，根据测试的菌株，效果和倍增差异很大。在本文中，我们在实验室可用的一组细胞上探索了体外 SARS-CoV-2 的物种特异性和组织嗜性，并确定了易支持 SARS-CoV-2 复制的人类和动物细胞系。我们的工作强调了在测试抗病毒分子和进行病理生理分析时测试多种菌株的重要性。如在 Caco-2 细胞中。有趣的是，根据测试的菌株，效果和倍增差异很大。在本文中，我们在实验室可用的一组细胞上探索了体外 SARS-CoV-2 的物种特异性和组织嗜性，并确定了易支持 SARS-CoV-2 复制的人类和动物细胞系。我们的工作强调了在测试抗病毒分子和进行病理生理分析时测试多种菌株的重要性。如在 Caco-2 细胞中。有趣的是，根据测试的菌株，效果和倍增差异很大。在本文中，我们在实验室可用的一组细胞上探索了体外 SARS-CoV-2 的物种特异性和组织嗜性，并确定了易支持 SARS-CoV-2 复制的人类和动物细胞系。我们的工作强调了在测试抗病毒分子和进行病理生理分析时测试多种菌株的重要性。