Object As a broad-spectrum fluoroquinolone antibiotic drug, ciprofloxacin (CIP) is frequently used in the treatment of a wide variety of infections. However, the residues of this antibiotic pose a big threat to the aquatic environment and human health. In this research, Lactobacillus reuteri WQ-Y1 with CIP degradation ability was screened and identified. Results L. reuteri WQ-Y1 with a degradation rate of 65.1% for 4 µg mL −1 CIP was screened from 17 lactic acid bacteria (LAB), and cytochrome P450 enzyme was confirmed to promote the degradation of CIP by L. reuteri WQ-Y1. Meanwhile, the CIP degradation rate were also higher in 48 h’ culture time when co-cultured with 1 mg/mL of glucose in the culture media. Furthermore, result also proved that fluoroquinolone antibiotics with the similar piperazine ring structures could be degraded by L. reuteri WQ-Y1. Conclusions L. reuteri WQ-Y1 could degrade fluoroquinolone antibiotics with the similar piperazine ring structure. However, future work still needs to be done on the confirmation of the key enzymes in the cytochrome P450 enzymes family in the biodegradation. Graphic Abstract The isolated ciprofloxacin-degrading strain L. reuteri WQ-Y1 had a CIP degradation rate of 65.1% at 24 hours, and one biodegradation metabolite was identified and proved to be an important metabolite of CIP from cytochrome P450 enzymes family hydrolysis with UPLC-MS/MS spectrograms approach.

中文翻译：

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具有环丙沙星降解能力的罗伊氏乳杆菌 WQ-Y1 的表征

目的 环丙沙星（CIP）作为一种广谱氟喹诺酮类抗生素药物，常用于治疗多种感染。然而，这种抗生素的残留对水生环境和人类健康构成了巨大威胁。本研究筛选并鉴定了具有CIP降解能力的罗伊氏乳杆菌WQ-Y1。结果从17个乳酸菌（LAB）中筛选出4 μg mL -1 CIP降解率为65.1%的罗伊氏乳杆菌WQ-Y1，证实细胞色素P450酶促进罗伊氏乳杆菌WQ-降解CIP Y1。同时，当与培养基中的 1 mg/mL 葡萄糖共培养时，CIP 降解率在 48 小时的培养时间内也更高。此外，结果还证明了具有相似哌嗪环结构的氟喹诺酮类抗生素可以被 L. 罗伊氏WQ-Y1。结论 L. reuteri WQ-Y1 可降解具有相似哌嗪环结构的氟喹诺酮类抗生素。但是，对于细胞色素P450酶家族在生物降解中的关键酶的确认，未来的工作仍然需要做。图文摘要分离得到的环丙沙星降解菌株罗伊氏乳杆菌WQ-Y1在24小时CIP降解率为65.1%，鉴定出一种生物降解代谢物，经UPLC- MS/MS 谱图方法。