The growing consumer demand for natural products led to an increasing interest in vanillin production by biotechnological routes. In this work, the biotechnological vanillin production by Amycolatopsis sp. ATCC 39116 is studied using ferulic acid as precursor, aiming to achieve maximized vanillin productivities. During biotech-vanillin production, the effects of glucose, vanillin and ferulic acid concentrations in the broth proved to be relevant for vanillin productivity. Concerning glucose, its presence in the broth during the production phase avoids vanillin conversion to vanillic acid and, consequently, increases vanillin production. To avoid the accumulation of vanillin up to a toxic concentration level, a multiple-pulse-feeding strategy is implemented, with intercalated vanillin removal from the broth and biomass recovery. This strategy turned out fruitful, leading to 0.46 g L⁻¹ h⁻¹ volumetric productivity of vanillin of and a production yield of 0.69 g_vanillin g_{ferulic acid}⁻¹, which are among the highest values reported in the literature for non-modified bacteria.

消费者对天然产品日益增长的需求导致对通过生物技术途径生产香兰素的兴趣日益浓厚。在这项工作中，Amycolatopsis的生物技术香兰素生产sp. 使用阿魏酸作为前体对 ATCC 39116 进行了研究，旨在实现最大的香草醛生产率。在生物技术香草醛生产过程中，肉汤中葡萄糖、香草醛和阿魏酸浓度的影响被证明与香草醛生产率相关。关于葡萄糖，它在生产阶段存在于肉汤中可避免香兰素转化为香兰素酸，从而增加香兰素的产量。为了避免香草醛积累到有毒浓度水平，实施了多脉冲饲喂策略，从肉汤中去除插入的香草醛并回收生物量。该策略结果是富有成效的，导致 0.46 g L ^-1 h ^-1香兰素的体积生产力和 0.69 g_香兰素的产量g_{阿魏 酸}^-1，这是文献中报道的非修饰细菌的最高值之​​一。