An aerobic, Gram-staining-positive, rod-shaped, endospore-forming and motile bacterial strain, designated SJY2 T , was isolated from the rhizosphere soil of tea plants ( Camellia sinensis var. assamica ) collected in the organic tea garden of the Jingmai Pu-erh tea district in Pu'er city, Yunnan, southwest China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Paenibacillus . The closest phylogenetic relative was Paenibacillus filicis DSM 23916 T (98.1% similarity). The major fatty acids (> 10% of the total fatty acids) were anteiso-C 15:0 and isoC 16:0 . The major respiratory quinone was MK-7 and the major polar lipid was diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. The peptidoglycan contained glutamic acid, serine, alanine and meso-diaminopimelic acid. Genome sequencing revealed a genome size of 6.71 Mbp and a G + C content of 53.1%. Pairwise determined whole genome average nucleotide identity (gANI) values and digital DNA–DNA hybridization (dDDH) values suggested that strain SJY2 T represents a new species, for which we propose the name Paenibacillus puerhi sp. nov. with the type strain SJY2 T (= CGMCC 1.17156 T = KCTC 43242 T ).

中文翻译：

---

普洱类芽孢杆菌 11 月，从普洱茶植物 (Camellia sinensis var. assamica) 的根际土壤中分离

从景迈有机茶园采集的茶树（Camellia sinensis var. assamica）根际土壤中分离到一株需氧、革兰氏染色阳性、棒状、能形成内生孢子且能运动的菌株，命名为SJY2 T。云南省普洱市普洱茶区。基于16S rRNA基因序列的系统发育分析表明该分离物属于类芽孢杆菌属。最接近的系统发育亲属是 Paenibacillus filicis DSM 23916 T（98.1% 相似性）。主要脂肪酸（> 总脂肪酸的 10%）是反异 C 15:0 和异 C 16:0。主要呼吸醌是MK-7，主要极性脂质是二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰单甲基乙醇胺。肽聚糖含有谷氨酸、丝氨酸、丙氨酸和内消旋二氨基庚二酸。基因组测序显示基因组大小为 6.71 Mbp，G + C 含量为 53.1%。成对确定的全基因组平均核苷酸同一性 (gANI) 值和数字 DNA-DNA 杂交 (dDDH) 值表明菌株 SJY2 T 代表一个新物种，我们建议将其命名为 Paenibacillus puerhi sp。十一月 与类型应变 SJY2 T (= CGMCC 1.17156 T = KCTC 43242 T)。