Ormocarpum sennoides (bone-knit) is a vulnerable medicinal plant species belongs to the family Leguminosae. The aim of the present study was to evaluate the influence of plant growth regulators (PGRs), additives (ascorbic acid, citric acid, adenine sulfate, and L-arginine), gelling agents (agar and phytagel™) and type of culture vessels on the quantitative and qualitative proliferation of shoots and the micro-morpho-anatomical state of the in vitro multiplied O. sennoides. The pre-treatment of single-node explants with antioxidants (citric acid and ascorbic acid) reduced phenolic exudates, and 93.8% bud breaking was observed on Murashige and Skoog’s (MS) medium with 1.5 mg L⁻¹ 6-benzylaminopurine (BAP). The highest rate of shoot enhancement (mean number of shoots) per explant (42.0 shoots with 6.3 cm length) was obtained on MS medium augmented with 0.5 mg L⁻¹ each of BAP and indole-3 acetic acid (IAA) with additives. Defoliation was observed in agar-gelled medium whereas; thick and sturdy shoots with healthy leaves were detected on phytagelled MS medium. The liquid medium was found unsuitable for shoot proliferation as the shoots experienced defoliation and hyperhydricity in cultures. The micro-shoots were rooted on half strength MS medium supplemented with 2.0 mg L⁻¹ indole-3 butyric acid (IBA) and hardened in a greenhouse. The leaves and shoots showed some characteristic anomalies in the cuticular membrane, epidermis, stomata, vein structures, chlorophyll development, hypertrophy in cortical cells, and lignifications in vascular elements when cultured in bottles capped with screw-caps. Such anomalies were absent with the shoots cultured in conical flasks capped with cotton plugs. The micro-morpho-anatomical evidences supported that MS medium gelled with phytagel™ containing additives with optimized PGRs in conical flasks could be the best physio-chemical parameters for qualitative and quantitative development of plantlets for the in vitro conservation of this vulnerable plant species.

森毛果（Ormocarpum sennoides）（骨编织）是一种易受伤害的药用植物，属于豆科。本研究的目的是评估植物生长调节剂（PGR），添加剂（抗坏血酸，柠檬酸，硫酸腺嘌呤和L-精氨酸），胶凝剂（琼脂和phytagel™）和培养容器类型对植物生长的影响。繁殖倍增O. sennoides的芽的数量和质量定性增殖以及微观形态解剖状态。用抗氧化剂（柠檬酸和抗坏血酸）预处理单节点外植体可减少酚类渗出液，在含1.5 mg L ^-1的Murashige和Skoog（MS）培养基上观察到93.8％的芽破裂。6-苄氨基嘌呤（BAP）。在外加0.5 mg L ^-1的BAP和吲哚3乙酸（IAA）和添加剂的MS培养基上，每个外植体（42.0个新芽，长6.3 cm长）的最高芽增强率（平均芽数）达到最高。在琼脂凝胶培养基中观察到落叶。在以phytagelled MS为基质的培养基上检测到带有健康叶片的粗壮茎。发现液体培养基不适合芽繁殖，因为芽在培养物中经历了脱叶和高水合。微芽植根于补充有2.0 mg L ^-1的半强度MS培养基上吲哚3丁酸（IBA），并在温室中硬化。在装有螺旋盖的瓶子中培养时，叶片和嫩芽在表皮膜，表皮，气孔，静脉结构，叶绿素发育，皮质细胞肥大和血管元素木质化方面表现出一些特征性异常。在用棉塞盖住的锥形瓶中培养的芽不存在这种异常。微观形态解剖学证据支持，在锥形瓶中用phytagel™凝胶化的含有PGtagel™添加剂的MS培养基具有优化的PGR，可能是定性和定量发育小植株的最佳生理化学参数，以用于该脆弱植物的体外保存。