Purpose

During infection, the host and the parasite “communicate” with each other through various molecules, including proteins. The aim of this study was to describe the excretory–secretory proteins from the helminth Schistocephalus solidus and its intermediate host, the three-spined stickleback Gasterosteus aculeatus L., which are likely to be involved in interactions between them.

Methods

Combined samples of washes from the G. aculeatus sticklebacks cavity infected with the S. solidus, and washes from the parasite surface were used as experimental samples, while washes from the uninfected fish body cavity were used as control. The obtained samples were analyzed using mass-spectrometry nLC–MS/MS.

Results

As a result of mass-spectrometry analysis 215 proteins were identified. Comparative quantitative analysis revealed significant differences in LFQ intensity between experimental and control samples for 20 stickleback proteins. In the experimental samples, we found an increase in the content of serpins, plasminogen, angiotensin 1–10, complement component C9, and a decrease in the content of triosephosphate isomerase, creatine kinase, fructose-biphosphate aldolase, superoxide dismutase, peroxidoxin-1, homocysteine-binding and fatty acid-binding proteins, compared to uninfected fish samples. In the experimental group washes, 30 S. solidus proteins were found, including malate dehydrogenase, annexin family proteins, serpins, peptidyl-prolyl cis–trans isomerase and fatty acid-binding protein.

Conclusions

Thus, the protein composition of washes from the helminth S. solidus surface and the body cavity of infected and uninfected stickleback G. aculeatus were studied. As a result, it was shown that various components of the immune defense system predominated in the washes of infected fish and helminths.

中文翻译：

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寄生虫和宿主之间的相互作用区：棘爪 Gasterosteus L. 体腔液的蛋白质分布。 感染 Cestode Schistocephalus solidus (Muller, 1776)

目的

在感染期间，宿主和寄生虫通过各种分子（包括蛋白质）相互“交流”。本研究的目的是描述蠕虫Schistocephalus solidus及其中间宿主三刺棘鱼Gasterosteus aculeatus L.的排泄-分泌蛋白，它们可能参与了它们之间的相互作用。

方法

从洗涤合并的样品G.棘孢刺鱼腔感染S.固相线从寄生虫表面，和洗涤液作为实验样品，而来自未感染鱼体腔洗涤液用作对照。使用质谱 nLC-MS/MS 分析获得的样品。

结果

作为质谱分析的结果，鉴定了215种蛋白质。比较定量分析揭示了 20 种棘鱼蛋白的实验样品和对照样品之间 LFQ 强度的显着差异。在实验样品中，我们发现丝氨酸蛋白酶抑制剂、纤溶酶原、血管紧张素 1-10、补体成分 C9 含量增加，磷酸丙糖异构酶、肌酸激酶、果糖二磷酸醛缩酶、超氧化物歧化酶、过氧化物酶 1 含量降低，同型半胱氨酸结合蛋白和脂肪酸结合蛋白，与未感染的鱼样品相比。在实验组洗涤中，发现了30种S.solidus蛋白，包括苹果酸脱氢酶、膜联蛋白家族蛋白、丝氨酸蛋白酶抑制剂、肽基-脯氨酰顺式-反式 异构酶和脂肪酸结合蛋白。

结论

因此，研究了来自蠕虫S.solidus表面和感染和未感染棘鱼G. aculeatus体腔的洗液的蛋白质组成。结果表明，免疫防御系统的各种成分在受感染的鱼和蠕虫的洗涤液中占主导地位。