Biochips have become a sophisticated analytical device in the fields of biochemical sensing and genetic analysis. However, the cumbersome preparation process and the high production cost limit the versatility of its application. Herein, we have developed an automated synthesis system for in situ preparation of biochip with peptide backbone based on the microfluidic mixer and micro reaction chamber. The microfluidic mixer was used as a key component to perform the real-time activation of the carboxylic groups, leading to an instant coupling reaction of monomers with high efficiency. The repeating synthesis procedure was realized without too much manual intervention with the help of flow control system based on programmable logical controller and LabVIEW. The real-time monitoring of synthesis process was realized using a low-cost solar cell coupled with simple ultraviolet absorption device. The photodeprotection experiment revealed that an exposure time of 4 min with 20 mW/cm ² ultraviolet (UV) light at 365nm was sufficient for the complete removal of 2-(2-nitrophenyl) propyloxycarbonyl (NPPOC) groups from the synthetic sites in N, N-dimethylformamide (DMF). The practical capability performance of this synthesis system was further demonstrated by the synthesis of four cycles of aminocaproic acid, and the stepwise yield of coupling was measured to be about 96%, which was comparable with the result from literature, and indicated that this system may provide a new alternative for low-cost in situ synthesis of biochip.

中文翻译：

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基于微流控混合器的多肽生物芯片自动原位合成系统

生物芯片已成为生化传感和遗传分析领域的精密分析设备。然而，繁琐的制备过程和高昂的生产成本限制了其应用的多功能性。在此，我们开发了一种自动化合成系统，用于就地基于微流体混合器和微反应室制备具有肽骨架的生物芯片。微流控混合器被用作实时活化羧基的关键部件，从而导致单体的高效偶联反应。借助基于可编程逻辑控制器和LabVIEW的流程控制系统，无需过多人工干预即可实现重复合成过程。使用低成本的太阳能电池和简单的紫外线吸收装置实现了合成过程的实时监控。光脱保护实验表明，在 20 mW/cm ²365nm 的紫外 (UV) 光足以从 N, N-二甲基甲酰胺 (DMF) 的合成位点完全去除 2-(2-硝基苯基) 丙氧羰基 (NPPOC) 基团。通过四次氨基己酸循环的合成进一步证明了该合成体系的实际能力性能，测得偶联的逐步产率约为96%，与文献结果相当，表明该体系可为低成本提供新的选择就地 生物芯片的合成。