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Phylogenetic relationships and DNA barcoding of nine endangered medicinal plant species endemic to Saint Katherine protectorate
Saudi Journal of Biological Sciences Pub Date : 2021-01-01 , DOI: 10.1016/j.sjbs.2020.12.043
Ahmed M. Hashim , Aishah Alatawi , Faris M. Altaf , Sameer H. Qari , Mohamed E. Elhady , Gamal H. Osman , Heba H. Abouseadaa

A high degree of endemism has been recorded for several plant groups collectively in Saint Katherine Protectorate (SKP) in the Sinai Peninsula. Nine endangered endemic plant species in SKP were selected to test the variable abilities of three different DNA barcodes; Riboluse-1,5- Biphosphate Carboxylase/Oxygenase Large subunit (rbcL), Internal Transcribed Spacer (ITS), and the two regions of the plastid gene (ycf1) as well as Start Codon Targeted (SCoT) Polymorphism to find the phylogenetic relationships among them. The three barcodes were generally more capable of finding the genetic relationships among the plant species under study, new barcodes were introduced to the National Centre for Biotechnology Information (NCBI) for the first time through our work. The barcode sequences were efficient in finding the genetic relationships between the nine species. However, SCoT polymorphism could only cluster plant species belonging to the same genus together in one group, but it could not cluster plant species belonging to the same families except for some primers solely. RbcL was the most easily amplified and identified barcode in eight out of the nine species at the species level and the ninth barcode to the genus level. ITS identified all the species to the genus level. Finally, ycf1 identified six out of the eight species, but it could not identify two of the eight species to the genus level.



中文翻译:

圣凯瑟琳保护地特有的9种濒危药用植物的亲缘关系和DNA条形码

西奈半岛的圣凯瑟琳保护区(SKP)集体记录了几种植物的高度流行。选择了SKP中的9种濒危特有植物物种,以测试三种不同DNA条形码的可变能力。Riboluse-1,5-二磷酸羧化酶/加氧酶大亚基(rbcL),内部转录间隔区(ITS)和质体基因的两个区域(ycf1)以及“起始密码子靶向(SCoT)多态性”以找到它们之间的系统发育关系。这三个条形码通常更有能力找到所研究植物物种之间的遗传关系,新条形码首次通过我们的工作引入了国家生物技术信息中心(NCBI)。条码序列可有效地发现这9个物种之间的遗传关系。然而,SCoT多态性只能将属于同一属的植物物种聚集在一起,而不能仅属于某些引物而将属于相同家族的植物物种聚集在一起。在物种水平上,RbcL是九种物种中八种中最容易扩增和鉴定的条形码,而在属水平上是第九个条形码。ITS识别了所有属的物种。最终,ycf1识别出八种中的六种,但无法识别到属水平的八种中的两种。

更新日期:2021-03-04
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