Nickel has been extensively used as a high work function metal because of its abundance, low cost, relatively non-toxic nature, and environmentally benign characteristics. However, it has rarely been extended in a form of immobilized composite, which is a practical strategy applicable for photocatalytic antimicrobial activities. In this study, a composite of nickel and TiO₂ (Ni/TiO₂) was prepared using a photodeposition method, and its antibacterial properties were investigated using Escherichia coli (E. coli). To optimize Ni/TiO₂ synthesis, the effect of various photodeposition conditions on antibacterial performance were investigated, such as the light irradiation time, metal content, TiO₂ crystalline structure, and presence or absence of electron donors (i.e., methanol). The optimized 2 wt% Ni/TiO₂ exhibited an antibacterial efficiency of 3.74 log within 7 min, which is more than 10-fold higher than that of pristine TiO₂ (2.54 log). Based on this optimized weight ratio, Ni/TiO₂ was immobilized on a steel mesh using an electrospray/thermal compression method, and its antibacterial performance was further assessed against E. coli, MS2 bacteriophage virus (MS2 phage), and a common pulmonary pathogen (Legionella thermalis, L. thermalis). Within 70 min, all target microorganisms achieved an inactivation that exceeded 4 log. Furthermore, the long-term stability and sustainable usability of the Ni/TiO₂ mesh were confirmed by performing more than 50 antibacterial evaluation cycles using E. coli. The results of this study facilitate the successful utilization of immobilized Ni/TiO₂ mesh in water disinfection applications.

镍由于其含量丰富，成本低，相对无毒的性质以及对环境有益的特性而被广泛用作高功函数金属。但是，它很少以固定复合材料的形式扩展，这是适用于光催化抗菌活性的实用策略。在这项研究中，使用光沉积法制备了镍和TiO ₂的复合材料（Ni / TiO ₂），并使用大肠杆菌（E. coli）研究了其抗菌性能。优化Ni / TiO ₂在合成过程中，研究了各种光沉积条件对抗菌性能的影响，例如光照射时间，金属含量，TiO ₂晶体结构以及是否存在电子给体（即甲醇）。优化的2 wt％Ni / TiO ₂在7分钟内显示出3.74 log的抗菌效率，比原始TiO ₂（2.54 log）高出10倍以上。基于此优化的重量比，使用电喷雾/热压缩方法将Ni / TiO ₂固定在钢网上，并进一步评估其对大肠杆菌，MS2噬菌体病毒（MS2噬菌体）和常见肺部病原体的抗菌性能（热军团菌，热乳杆菌。在70分钟内，所有目标微生物的灭活均超过4 log。此外，通过使用大肠杆菌进行了50多次抗菌评估循环，证实了Ni / TiO ₂筛网的长期稳定性和可持续使用性。这项研究的结果促进了固定化Ni / TiO ₂筛孔在水消毒应用中的成功利用。