Indian Journal of Microbiology ( IF 2.1 ) Pub Date : 2021-01-01 , DOI: 10.1007/s12088-020-00914-2 R Ansiliero 1 , J M L N Gelinski 1 , Q L Samistraro 1 , C M Baratto 1 , C A Almeida 1 , C Locatelli 2
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Abstract
Phenotyping based on conventional microbiological, physiological, and molecular analysis by using ARDRA technique was developed with the aim to assess the pathogenic microbial load associated with different stages of the periodontal disease. In addition, in the face of the global issue of antimicrobial resistance, the isolated bacterial strains were evaluated for their antibiotic susceptibility profile. The pathogenic bacterial community was predominantly of Gram-negative strains (66.66%). The most common species were Citrobacter freundii, Bacillus sp., Raoutella sp., Klebsiella ozaenae and Pseudomonas sp. However, except for the healthy control group, Staphylococcus spp. was isolated from all stages of periodontitis. Multidrug resistance to beta-lactam antibiotics was observed for Streptococcus pneumoniae, Raoutella sp. and Enterococcus avium. Here, we verify a statistically significant relationship between periodontitis stages and the diversity of the bacterial community. Patients with periodontitis showed a more diverse and numerous bacterial community compared to healthy patients. In this sense, we reinforce that biofilms that harbour multidrug-resistant bacteria are a major concern in relation to restoring patient health. Thus, prophylactic measures for maintaining oral health are still the best option for reduce the risk of disease.
Graphic Abstract
中文翻译：
与牙周炎相关的病原微生物谱和抗生素耐药性
摘要
使用 ARDRA 技术开发了基于常规微生物学、生理学和分子分析的表型分析，旨在评估与牙周病不同阶段相关的病原微生物负荷。此外，面对全球抗菌素耐药性问题，对分离的细菌菌株的抗生素敏感性进行了评估。病原菌群落以革兰氏阴性菌为主（66.66%）。最常见的物种是弗氏柠檬酸杆菌、芽孢杆菌属、劳特氏菌属、克雷伯氏菌和假单胞菌属。然而，除了健康对照组，葡萄球菌spp. 从牙周炎的各个阶段分离出来。观察到肺炎链球菌、Raoutella sp.对β-内酰胺类抗生素产生多药耐药性。和鸟肠球菌。在这里，我们验证了牙周炎阶段与细菌群落多样性之间的统计学显着关系。与健康患者相比，牙周炎患者表现出更加多样化和数量众多的细菌群落。从这个意义上说，我们强调含有耐多药细菌的生物膜是恢复患者健康的主要问题。因此，维持口腔健康的预防措施仍然是降低疾病风险的最佳选择。
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