LncRNA HCP5 has been confirmed to play crucial roles in many types of cancers. However, the role of lncRNA HCP5 in regulating the occurrence and development of gastric cancer (GC) remains unknown. In the current study, we aimed to investigate the precise effects of lncRNA HCP5 on cell proliferation, migration and invasion and molecular mechanisms in gastric cancer. Using RT-qPCR analysis, we found that lncRNA HCP5 was differentially expressed in GC cell lines. CCK-8, wound healing and transwell assay indicated that the proliferation, migration and invasion of gastric cancer cells were inhibited by downregulation of lncRNA HCP5 and lncRNA HCP5 overexpression exhibited the opposite effects in gastric cancer cells. Mechanistically, RNA binding protein immunoprecipitation and dual luciferase reporter assay confirmed the interaction between lncRNA HCP5 and DDX21. The effects of lncRNA HCP5 overexpression the proliferation, migration and invasion of GC cells were partly rescued by DDX21 silencing. Taken together, downregulation of lncRNA HCP5 exerted inhibitory effects on GC cell proliferation, migration and invasion through modulation of DDX21 expression, demonstrating the function of lncRNA HCP5 and DDX21 in GC progression.

LncRNA HCP5 已被证实在多种癌症中发挥关键作用。然而，lncRNA HCP5在调控胃癌（GC）发生发展中的作用仍不清楚。在本研究中，我们旨在研究 lncRNA HCP5 对胃癌细胞增殖、迁移和侵袭以及分子机制的精确影响。使用 RT-qPCR 分析，我们发现 lncRNA HCP5 在 GC 细胞系中差异表达。CCK-8、伤口愈合和transwell试验表明，lncRNA HCP5的下调抑制了胃癌细胞的增殖、迁移和侵袭，而lncRNA HCP5过表达在胃癌细胞中表现出相反的作用。机械地，RNA 结合蛋白免疫沉淀和双荧光素酶报告基因测定证实了 lncRNA HCP5 和 DDX21 之间的相互作用。LncRNA HCP5过表达对GC细胞增殖、迁移和侵袭的影响部分被DDX21沉默所挽救。总之，lncRNA HCP5的下调通过调节DDX21的表达对GC细胞增殖、迁移和侵袭产生抑制作用，证明了lncRNA HCP5和DDX21在GC进展中的作用。