Background There is increasing evidence that circular RNA (circRNA) disorders have an impact on the progression of various malignancies. The expression characteristics, function and underlying mechanism of circ_0001247 in cervical cancer (CC) have not been confirmed. Methods GSE147483 datasets of circRNAs expression in CC cell line and normal cervical cell line were retrieved from GEO database, and the circRNA with significant difference was selected; circ_0001247, miR-1270, and Zinc finger E-box binding homeobox 2 (ZEB2) expressions in CC tissues and cell lines were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) assay; cell counting kit-8 (CCK-8) assay and BrdU assay were applied to monitor the proliferative ability of CC cells; Transwell assay was conducted to examine the migration and invasion of CC cells, and flow cytometry was used to evaluate the apoptosis; Western blot assay was adopted to detect ZEB2 protein expressions; dual-luciferase report gene assay was used to verify the targeting relationship between circ_0001247 and miR-1270, and miR-1270 and the 3’UTR of ZEB2. Results Analysis of GSE147483 suggested that circ_0001247 could probably be an oncogenic circRNA in CC. Compared with that in adjacent tissues and normal cervical epithelial cells, circ_0001247 expression in CC tissues and cell lines was significantly increased; knocking down circ_0001247 expression could inhibit the proliferation and metastasis of CC cells, and promote apoptosis, while circ_0001247 overexpression worked oppositely; circ_0001247 sponged miR-1270 in CC cells; miR-1270 diminished the promoting effect of circ_0001247 by inactivating the ZEB2. Conclusion Circ_0001247 promotes progression of CC by sponging miR-1270 to upregulate ZEB2 expression level.

中文翻译：

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Circ_0001247 作为 miR-1270 海绵通过上调 ZEB2 表达水平加速宫颈癌进展

背景 越来越多的证据表明环状 RNA (circRNA) 疾病对各种恶性肿瘤的进展有影响。circ_0001247在宫颈癌（CC）中的表达特征、功能和潜在机制尚未得到证实。方法从GEO数据库中检索CC细胞系和正常宫颈细胞系circRNAs表达的GSE147483数据集，选取差异显着的circRNA；circ_0001247、miR-1270和锌指E-box结合同源框2（ZEB2）在CC组织和细胞系中的表达通过定量实时聚合酶链反应（qRT-PCR）分析；应用细胞计数试剂盒-8（CCK-8）和BrdU检测来监测CC细胞的增殖能力；进行 Transwell 实验以检查 CC 细胞的迁移和侵袭，流式细胞术评估细胞凋亡；采用Western blot检测ZEB2蛋白表达；双荧光素酶报告基因检测用于验证circ_0001247与miR-1270、miR-1270与ZEB2的3'UTR之间的靶向关系。结果 GSE147483 的分析表明 circ_0001247 可能是 CC 中的致癌 circRNA。与癌旁组织和正常宫颈上皮细胞相比，circ_0001247在CC组织和细胞系中的表达显着增加；敲低circ_0001247的表达可以抑制CC细胞的增殖和转移，促进细胞凋亡，而circ_0001247的过表达则相反；circ_0001247 在 CC 细胞中吸附 miR-1270；miR-1270 通过灭活 ZEB2 减弱了 circ_0001247 的促进作用。