Syzygium jambos (Myrtales, Myrtaceae) trees in Hawaii are severely affected by a rust disease caused by Austropuccinia psidii (Pucciniales, Sphaerophragmiaceae), but they are commonly co-infected with species of Cryphonectriaceae (Diaporthales). In this study, S. jambos and other trees in the Myrtales were examined on three Hawaiian Islands for the presence of Cryphonectriaceae. Bark samples with fruiting bodies were collected from infected trees and fungi were isolated directly from these structures. Pure cultures were produced and the fungi were identified using DNA sequence data for the internal transcribed spacer (ITS) region, part of the β-tubulin (BT1) gene and the transcription elongation factor-1α (TEF1) gene. Five species in three genera of Cryphonectriaceae were identified from Myrtaceae tree samples. These included Chrysoporthe deuterocubensis, Microthia havanensis and three previously-unknown taxa described here as Celoporthe hauoliensis sp. nov., Cel. hawaiiensis sp. nov. and Cel. paradisiaca sp. nov. Representative isolates of Cel. hauoliensis, Cel. hawaiiensis, Cel. paradisiaca, Chr. deuterocubensis and Mic. havanensis were used in artificial inoculation studies to consider their pathogenicity on S. jambos. Celoporthe hawaiiensis, Cel. paradisiaca and Chr. deuterocubensis produced lesions on young S. jambos trees in inoculation trials, suggesting that, together with A. psidii, they may contribute to the death of trees. Microsatellite markers were subsequently used to consider the diversity of Chr. deuterocubensis on the Islands and thus to gain insights into its possible origin in Hawaii. Isolates of this important Myrtaceae and particularly Eucalyptus pathogen were found to be clonal. This provides evidence that Chr. deuterocubensis was introduced to the Hawaiian Islands as a single introduction, from a currently unknown source.

中文翻译：

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与夏威夷受锈病感染的蒲桃有关的隐藻科


夏威夷的桃金娘（桃金娘目、桃金娘科）树受到由 Austropuccinia psidii（柄锈菌目、Sphaerophragmiaceae）引起的锈病的严重影响，但它们通常与 Cryphonectriaceae（Diaporthales）物种同时感染。在这项研究中，我们对夏威夷三个岛屿上的香桃木和其他桃金娘科树木进行了检查，以确定其是否存在 Cryphonectriaceae。从受感染的树木中收集带有子实体的树皮样本，并直接从这些结构中分离出真菌。产生纯培养物，并使用内转录间隔区 (ITS) 区域、部分 β-微管蛋白 (BT1) 基因和转录延伸因子 1α (TEF1) 基因的 DNA 序列数据来鉴定真菌。从桃金娘科树样本中鉴定出隐菌科三个属的五个物种。其中包括 Chrysoporthe deuterocubensis、Microthia havanensis 和三个以前未知的类群，此处描述为 Celoporthe hauoliensis sp。十一月，塞尔。夏威夷草十一月和塞尔。帕拉迪西亚卡十一月Cel 的代表性分离株。夏威夷州夏威夷，塞尔。帕拉迪西亚卡，Chr。 deuterocubensis 和 Mic。 havanensis 被用于人工接种研究，以考虑其对 S. jambos 的致病性。夏威夷赛罗波罗 (Celoporthe hawaiiensis)，Cel。 paradisiaca 和 Chr。在接种试验中，deuterocubensis 在幼树上产生了损伤，这表明它们与 A. psidii 一起可能导致树木死亡。随后使用微卫星标记来考虑 Chr 的多样性。岛屿上的 deuterocubensis ，从而深入了解其可能起源于夏威夷。这种重要的桃金娘科，特别是桉树病原体的分离株被发现是克隆的。这提供了证据表明科。 deuterocubensis 作为单一引种被引入夏威夷群岛，来源目前未知。