当前位置: X-MOL 学术Environ. Entomol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Metarhizium Entomopathogenic Fungi Against the Beetle Brontispa longissima (Coleoptera: Hispidae): Isolation and Species Identification
Environmental Entomology ( IF 1.8 ) Pub Date : 2020-12-31 , DOI: 10.1093/ee/nvaa156
Tao Lian 1 , Hua-long Qiu 1 , Chang-sheng Qin 1 , Eduardo G P Fox 2 , Dan-yang Zhao 1 , Tian-song Fang 3 , Hua Yang 1 , Long-yan Tian 1 , Jin-zhu Xu 1
Affiliation  

Seven entomopathogenic fungi strains (M1-7) were isolated from field-obtained dead coconut hispine beetles Brontispa longissima (Gestro), identified to species, and bioassayed for their pathogenicity. According to ITS sequences, all isolates belong in the genus Metarhizium, mainly M. flavoviride and M. anisopliae. Measured median lethal times (LT50) of 1×107 conidia/ml of M1-7 against fourth-instar B. longissima larvae within 15 d following exposure were, respectively: 5.43, 10.64, 11.26, 10.93, 6.62, 4.73, and 5.95 d. The isolate M6 yielded the highest mortality to fourth-instar larvae, and was thus selected to be tested against other larval instars and adults of B. longissima, after Time-Dose-Mortality (TDM) models. M6 proved more pathogenic against larvae than adults. The obtained bioassays data produced a good fit to the TDM models, yielding estimated LC50 and LT50 for each of the tested developmental stages of B. longissima. Both the obtained dose (β) and time effect (ri) parameters from TDM models suggest that first-instar larvae are the most susceptible life stage of the pest insect, while adults are more resistant to M6 infection. Calculated LC50 values were, respectively, 1.23×103 and 1.15×106 conidia/ml for first-instar larvae and adults, on the 15th day following M6 inoculation. Estimated LT50 were 3.3 and 5.9 d for first-instar larvae and adults, respectively, at 1×108 conidia/ml. Taken together, these results would suggest Metarhizium M6 as an option for the biological control of B. longissima in the field.

中文翻译:

对抗甲虫 Brontispa longissima(鞘翅目:Hispidae)的绿僵菌昆虫病原真菌:分离和物种鉴定

七株昆虫病原真菌菌株 (M1-7) 从田间获得的死椰子甲虫 Brontispa longissima (Gestro) 中分离出来,对物种进行了鉴定,并对其致病性进行了生物测定。根据 ITS 序列,所有分离株都属于绿僵菌属,主要是黄绿分枝杆菌和绿僵菌属。在暴露后 15 d 内测得的 1×107 分生孢子/ml M1-7 对四龄长双歧杆菌幼虫的中位致死时间 (LT50) 分别为:5.43、10.64、11.26、10.93、6.62、4.795 和 d . 分离株 M6 对四龄幼虫的死亡率最高,因此在时间-剂量-死亡率 (TDM) 模型之后,被选择用于针对其他幼虫和最长 B. longissima 的成虫进行测试。M6 证明对幼虫比成虫更具致病性。获得的生物测定数据很好地拟合了 TDM 模型,为 B. longissima 的每个测试发育阶段产生估计的 LC50 和 LT50。从 TDM 模型获得的剂量 (β) 和时间效应 (ri) 参数都表明,一龄幼虫是害虫最易感的生命阶段,而成虫对 M6 感染的抵抗力更强。在 M6 接种后的第 15 天,计算的 LC50 值分别为 1.23×103 和 1.15×106 个分生孢子/ml,对于一龄幼虫和成虫。一龄幼虫和成虫的 LT50 估计值分别为 3.3 和 5.9 天,分生孢子为 1×108/ml。综上所述,这些结果表明绿僵菌 M6 可作为田间 B. longissima 生物防治的一种选择。从 TDM 模型获得的剂量 (β) 和时间效应 (ri) 参数都表明,一龄幼虫是害虫最易感的生命阶段,而成虫对 M6 感染的抵抗力更强。在 M6 接种后的第 15 天,计算的 LC50 值分别为 1.23×103 和 1.15×106 个分生孢子/ml,对于一龄幼虫和成虫。一龄幼虫和成虫的 LT50 估计值分别为 3.3 天和 5.9 天,为 1×108 分生孢子/毫升。综上所述,这些结果表明绿僵菌 M6 可作为田间 B. longissima 生物防治的一种选择。从 TDM 模型获得的剂量 (β) 和时间效应 (ri) 参数都表明,一龄幼虫是害虫最易感的生命阶段,而成虫对 M6 感染的抵抗力更强。在 M6 接种后的第 15 天,计算的 LC50 值分别为 1.23×103 和 1.15×106 个分生孢子/ml,对于一龄幼虫和成虫。一龄幼虫和成虫的 LT50 估计值分别为 3.3 和 5.9 天,分生孢子为 1×108/ml。综上所述,这些结果表明绿僵菌 M6 可作为田间 B. longissima 生物防治的一种选择。M6接种后第15天。一龄幼虫和成虫的 LT50 估计值分别为 3.3 和 5.9 天,分生孢子为 1×108/ml。综上所述,这些结果表明绿僵菌 M6 可作为田间 B. longissima 生物防治的一种选择。M6接种后第15天。一龄幼虫和成虫的 LT50 估计值分别为 3.3 和 5.9 天,分生孢子为 1×108/ml。综上所述,这些结果表明绿僵菌 M6 可作为田间 B. longissima 生物防治的一种选择。
更新日期:2020-12-31
down
wechat
bug