Exploring metabolic pathways is one of the key techniques for developing highly productive microbes for the bioproduction of chemical compounds. To explore feasible pathways, not only examining a combination of well-known enzymatic reactions but also finding potential enzymatic reactions that can catalyze the desired structural changes are necessary. To achieve this, most conventional techniques use manually predefined-reaction rules, however, they cannot sufficiently find potential reactions because the conventional rules cannot comprehensively express structural changes before and after enzymatic reactions. Evaluating the feasibility of the explored pathways is another challenge because there is no way to validate the reaction possibility of unknown enzymatic reactions by these rules. Therefore, a technique for comprehensively capturing the structural changes in enzymatic reactions and a technique for evaluating the pathway feasibility are still necessary to explore feasible metabolic pathways.

中文翻译：

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使用化学潜空间的可行代谢途径探索技术

探索代谢途径是开发用于化学化合物生物生产的高产微生物的关键技术之一。为了探索可行的途径，不仅需要检查众所周知的酶促反应的组合，而且还需要寻找可以催化所需结构变化的潜在酶促反应。为了实现这一点，大多数常规技术使用手动预定义的反应规则，然而，它们不能充分发现潜在的反应，因为常规规则不能全面表达酶促反应前后的结构变化。评估所探索途径的可行性是另一个挑战，因为无法通过这些规则来验证未知酶促反应的反应可能性。所以，