Abiotic stresses, which at the molecular level leads to oxidative damage, are major determinants of crop yield loss worldwide. Therefore, considerable efforts are directed towards developing strategies for their limitation and mitigation. Here the superoxide-inducing agent paraquat (PQ) was used to induce oxidative stress in the model species Arabidopsis thaliana and the crops tomato and pepper. Pre-treatment with the biostimulant SuperFifty (SF) effectively and universally suppressed PQ-induced leaf lesions, H₂O₂ build up, cell destruction and photosynthesis inhibition. To further investigate the stress responses and SF-induced protection at the molecular level, we investigated the metabolites by GC-MS metabolomics. PQ induced specific metabolic changes such as accumulation of free amino acids (AA) and stress metabolites. These changes were fully prevented by the SF pre-treatment. Moreover, the metabolic changes of the specific groups were tightly correlating with their phenotypic characteristics. Overall, this study presents physiological and metabolomics data which shows that SF protects against oxidative stress in all three plant species.

中文翻译：

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一种来自Ascophyllum nodosum的生物刺激剂可保护模型和农作物免受氧化胁迫

在分子水平上导致氧化损伤的非生物胁迫是全世界作物产量损失的主要决定因素。因此，大量的努力致力于开发限制和缓解策略。在这里，超氧化物诱导剂百草枯（PQ）用于在拟南芥模型种以及作物番茄和胡椒中诱导氧化胁迫。用生物刺激剂SuperFifty（SF）进行的预处理可有效且普遍抑制PQ诱导的叶片损伤H ₂ O ₂积累，细胞破坏和光合作用抑制。为了进一步研究分子水平上的应激反应和SF诱导的保护作用，我们通过GC-MS代谢组学研究了这些代谢物。PQ引起特定的代谢变化，例如游离氨基酸（AA）的积累和应激代谢产物。SF预处理完全防止了这些变化。此外，特定人群的代谢变化与其表型特征密切相关。总的来说，这项研究提供了生理和代谢组学数据，这些数据表明，SF可以保护所有三种植物免受氧化胁迫。