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Correction to Efficient Site-Specific Prokaryotic and Eukaryotic Incorporation of Halotyrosine Amino Acids into Proteins
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2020-12-31 , DOI: 10.1021/acschembio.0c00951
Hyo Sang Jang , Xiaodong Gu , Richard B. Cooley , Joseph J. Porter , Rachel L. Henson , Taylor Willi , Joseph A. DiDonato , Stanley L. Hazen , Ryan A. Mehl

During deposition into Addgene, additional off-target mutations in the Mb haloTyr C6 aaRS were identified at sites 13, 82, and 265. Table 1 and Figure 1 report the correct sequence information for the functional aaRS’s used in bacterial and eukaryotic expression systems. Functional plasmids are now available from Addgene (numbers are listed in Table 1). Figure 1 contains the corrected sequences for haloTyr C6 aaRS: bacterial (DNA), bacterial (translated protein), eukaryotic (DNA, with N-terminal nuclear export sequence and FLAG tag), and eukaryotic (translated protein, DNA, with N-terminal nuclear export sequence and FLAG tag). Figure 1. Corrected sequences for haloTyr C6 aaRS. This article has not yet been cited by other publications. Figure 1. Corrected sequences for haloTyr C6 aaRS.

中文翻译:

纠正到位点特异性原核糖核酸和真核糖核酸氨基酸的真核生物。

在沉积到Addgene中的过程中,在位点13、82和265处发现了Mb haloTyr C6 aaRS中的其他脱靶突变。表1和图1报告了细菌和真核表达系统中使用的功能性aaRS的正确序列信息。现在可从Addgene获得功能性质粒(编号列于表1)。图1包含针对haloTyr C6 aaRS的校正序列:细菌(DNA),细菌(翻译的蛋白),真核生物(DNA,带有N端核输出序列和FLAG标签)和真核生物(翻译的蛋白,DNA,具有N端)核输出序列和FLAG标签)。图1. haloTyr C6 aaRS的校正序列。本文尚未被其他出版物引用。图1. haloTyr C6 aaRS的校正序列。
更新日期:2021-01-15
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